【摘要】 在广西香蕉种植区发现一种引起香蕉黑斑病的新病原。通过对病原菌分离培养、致病性测定、形态学观察、分子生物学鉴定及对该病原菌生物学特性进行初步研究,表明引起该病害的病原菌为芒果球座菌(Guignardia mangiferae A.J.Roy)。该病原菌菌落为深橄榄色,产生棒状子囊、梭形子囊孢子及倒梨形分生孢子;其最适生长温度为28℃,最适pH值为6;病原菌生长较好的碳源为果糖和阿拉伯糖,氮源为蛋白胨和酵母膏;在全光条件下病原菌菌丝扩展速度最快;菌丝的致死温度为54℃。由G.mangiferae引起的香蕉黑斑病,在国内外为首次报道。更多还原

【Abstract】 A new black spot disease was observed on the banana cultivar ’Williams’(AAA,Cavendish) in Guangxi province,China.The pathogen was isolated from the infected banana leaves and cultured.Based on the analysis of its morphology,biological characteristics,pathogenicity and ITS sequence,the pathogen was identified as Guignardia mangiferae A.J.Roy.Colonies of the pathogen on PDA were dark olive,and the ascus was clavate with stipe,ascospores were fusiform and conidia were obpyriform.G.mangiferae could grow with an optimal condition at 28℃,pH 6.The best carbon sources for the pathogen growth were fructose and arabinose,and the best nitrogen sources were peptone and yeast extract.The best cultural condition for mycelial growth was 24h under light.The lethal temperature was 54℃ for hypha of G.mangiferae.To the best of our knowledge,this is the first report of black spot on banana caused by G.mangiferae in the world.更多还原