【摘要】 目的通过检测程序性细胞死亡因子1(PD-1)及其配体1(PD-L1)在自身免疫性肝炎(AIH))患者不同分期肝组织中的表达情况,探讨其在AIH发病机制中的作用。方法收集43例AIH患者(包括活动期31例和缓解期12例)肝组织,并选择9例健康者为对照组。应用免疫组织化学方法结合图像定量分析系统检测肝组织内PD-1及PD-L1的表达情况,并比较不同病情分期PD-1及PD-L1表达的差异。结果 AIH患者肝组织PD-1为(10.12±5.31)%,高于对照组(1.74±0.37)%,(P<0.05),AIH活动期肝组织PD-1为(15.68±6.31)%,高于缓解期(6.24±3.25)%,(P<0.05)。AIH患者肝组织PD-L1为(7.45±4.29)%,高于对照组(1.48±0.32)%,(P<0.05);AIH活动期肝组织PD-L1为(9.47±4.67)%,高于缓解期(4.78±2.43)%(均P<0.05)。结论 PD-1及其配体PD-L1在AIH患者肝组织中高表达,提示其可能参与了AIH的发病机制。更多还原

【Abstract】 Objective To investigate the expressions of programmed death 1(PD-1)and programmed death ligand 1(PD-L1)in hepatic tissues of patients with autoimmune hepatitis(AIH),and to explore their roles in the pathogenesis of AIH.Methods The expressions of PD-1 and PD-L1 in hepatic tissues from43 AIH patients and 9 healthy controls were detected by immunohistochemistry combined with computer image quantitative analysis.Results The PD-1 and PD-L1 expressions in hepatic tissues of AIH patients were 10.12±5.31% and 7.45±4.29%,respectively,which were significantly higher than those in controls(1.74±0.37% and 1.48±0.32%,respectively).The expressions of PD-1 and PD-L1 in hepatic tissues of AIH patients at active stage were 15.68±6.31% and 7.45±4.29%,respectively,which were higher than those in AIH patients in remission(6.24±3.25% and 4.78±2.43%,respectively)and healthy controls(1.74±0.37% and 1.48±0.32%,respectively).Conclusion PD-1 and PD-L1 are overexpressed in hepatic tissues of AIH patients,which reveals that PD-1 and its ligand might play an important role in the pathogenesis of AIH.更多还原