【摘要】 目的探索人参皂苷Rg5对人食管癌细胞Eca-109的作用及机制。方法利用不同浓度人参皂苷Rg5在体外对人食管癌Eca109细胞作用不同时间,采用CCK-8法检测细胞抑制率,Annexin V-FITC/PI双染色后流式细胞仪检测细胞凋亡率,PI单染法检测细胞周期。结果在浓度分别为50、100μmol/L的人参皂苷Rg5作用人食管癌细胞24h后出现明显形态学变化,同时处于S期及G2/M期细胞较对照组明显增多,分别为53.08%、25.84%和58.44%、31.48%,流式细胞仪检测早期凋亡率分别为11.45%、30.13%。结论人参皂苷Rg5能抑制食管癌Eca-109细胞增殖,其机制可能为增加细胞早期凋亡,引起细胞周期滞留。更多还原

【Abstract】 Objective To explore the effect and mechanism of ginsenoside Rg5 on human esophageal cancer cell line Eca-109.Methods The inhibitory rate of human esophageal cancer cell line Eca109 in vitro was measured by CCK-8method.The apoptosis rate of cells was detected by flow cytometry after Annexin V-FITC/PI double staining.Cell cycle.Results After treated with ginsenoside Rg5 at concentrations of 50 and 100μmol/L for 24 h,the morphological changes were observed,and the cells in S phase and G2/M phase were significantly higher than those in the control group(53.08%,25.84%% and 58.44%,31.48% respectively(P<0.05).The early apoptotic rates were 11.45% and30.13% respectively by flow cytometry(P<0.05).Conclusion Ginsenoside Rg5 can inhibit the proliferation of esophageal cancer Eca-109 cells,and its mechanism may be to increase the early apoptosis of cells,causing cell cycle arrest.更多还原