【摘要】 为了建立猪传染性胸膜肺炎放线杆菌血清6型分子鉴定方法,本研究根据胸膜肺炎放线杆菌从编码荚膜多糖的碱基序列设计1对引物,扩增特异性的720 bp核酸片段。结果表明,以APP为模板,均能扩增出与预期一致的1条720 bp核酸片段,所得PCR产物经测序,与Gen Bank已发表的胸膜肺炎放线杆菌血清型6型的同源性达99%以上。本研究建立了PCR检测猪传染性胸膜肺炎放线杆菌血清6型分子鉴定方法,该方法的建立为猪传染性胸膜肺炎放线杆菌病的诊断和防治及鉴定提供了基础。更多还原

【Abstract】 In order to establish a method for the identification of Actinobacillus pleuropneumoniae serotype 6,a pair of primerswas designed according to the APP from the nucleotide sequence encoding the capsular polysaccharide for the 720 bp amplificationof 720 bp specific nucleic acid fragment. The results showed that the APP as a template,could be amplified in line with expecta-tions of the 720 bp fragment.The PCR products were then sequenced,and had a homology of 99% with the sequences published inGen Bank of APP serotype 6. The establishment of this method provides a basis for diagnosis and prevention and identification ofporcine Actinobacillus pleuropneumoniae.更多还原