【摘要】 为了评估秦岭地区珍稀野生动物的毕氏肠微孢子虫(Enterocytozoon bieneusi)的人兽共患风险,本研究采用PCR技术和多位点序列分型技术对秦岭地区5种珍稀动物毕氏肠微孢子虫的感染情况及基因型/亚型进行了分析。在采集的22份新鲜粪便中检测到7个阳性分离株,其中斑羚源5个,长颈鹿源1个,麂子源1个。基于ITS位点基因分型发现所有分离株均为人兽共患基因型,包括6个已报道的基因型D和1个尚未报道的D-new。多位点序列分型技术发现秦岭地区珍稀野生动物的毕氏肠微孢子虫存在遗传多样性,7个分离株在MS1、MS3、MS4和MS7位点分别有3、1、2、2个亚型。更多还原

【Abstract】 In order to determine the genotypes and subtypes of Enterocytozoon bieneusi in wildlife in Qinling Mountains,22 fecal specimens were collected and detected by molecular biological technique based on PCR and multilocus sequence typing.Seven specimens were positive in E.bieneusi in goral(n=5),giraffe(n=1)and barking deer(n=1).Genotyping at ITS locus and phylogenetic analysis indicated one known E.bieneusi genotype D and one novel E.bieneusi genotype D-new,both are zoonotic parasites.Multilocus sequence typing showed 3,1,2,2haplotypes at MS1,MS3,MS4,MS7 loci,respectively.The results in the present study have significant implication in prevention and control of zoonotic E.bieneusi infection.更多还原