【摘要】 目的研究发酵法L-丙氨酸对大鼠的亚慢性毒性和最大无作用剂量。方法依据《食品安全性毒理学评价程序和方法》进行试验,采用4周龄清洁级健康离乳SD大鼠96只,雌雄各半,随机分为4组,分别给予发酵法L-丙氨酸0.00、1.25、2.50、5.00 g/kg BW掺入基础饲料连续喂饲90 d。第45天眼内眦静脉采血进行血常规检测;第90天采血测定血常规及生化指标,取肝、脾、肾、睾丸、卵巢称重并计算其脏体比,并对大脑、小脑、心、胸腺、肺、肝、肾、肾上腺、脾、胃、十二指肠、睾丸、卵巢、子宫等进行病理学检查。结果各剂量组动物活动、生长正常,末期体重、总进食量、总食物利用率,以及脏器绝对重量、脏体比与对照组相比差异均无统计学意义(P>0.05);试验中、末期血常规和血生化指标均未发现有意义的异常改变;病理学检查未见与受试物有关的病理改变。结论发酵法L-丙氨酸对雌、雄大鼠亚慢性毒性试验未观察到有害作用剂量(NOAEL)分别为5.56、4.58 g/kg BW。更多还原

【Abstract】 Objective To evaluate the sub-chronic oral toxicity and maximum no observed adverse effect level( NOAEL)of L-alanine from fermentation broth in Sprague-Dawley( SD) strain rats. Methods According to procedures and methods for toxicological assessment of food( 2003 edition),a total of 96 4-week-age cleaning grade weanling SD rats were divided randomly into 4 groups,12 male rats and 12 female rats in each group. Rats were fed the diet containing 0. 00,1. 25,2. 50 and 5. 00 g / kg BW L-alanine from fermentation broth for 90 consecutive days respectively. On the 45 thday,blood samples collected from inner canthus veniplex were used for complete blood count. At the end of the experiment,samples of blood were collected for hematological and biochemical analysis,respectively. Livers,spleens,kidneys and testicles( ovariesy) from all rats were weighed,furthermore,and the ratio of these organs to body weight was determined. The brain( including cerebrum,cerebellum and pons cerebelli),heart,thymus,lung,liver,kidneys,adrenal gland,spleen,stomach,duodenum,testicles,ovariesy and uterus( horn and cervix) were removed for histopathological examinations.Results The growth and development of all rats in four groups generally performed well with normal growth and development. There were no significant differences between three experimental groups and the control group( P > 0. 05),including the final body weight,total food intake,total food utilization,the cardinal organs / tissues’ wet weight and organ coefficient. No sample-related significant changes were observed on haematology and serum biochemistry parameters at the mid or final term. Histopathological examinations showed no damage caused by L-alanine from fermentation broth,except for a few of the common spontaneous lesions. Conclusion The NOAEL of male and female SD rats in oral sub-chronic toxicity study for L-alanine from fermentation broth were 4. 58,5. 56 g / kg BW respectively.更多还原