【摘要】 目的通过对食物中毒病原菌的分离鉴定,为查明中毒原因提供科学依据。方法分离鉴定和毒性试验按照国家标准方法 WS/T 12—1996《椰毒假单胞菌酵米面亚种食物中毒诊断标准及处理原则》和GB/T 4789.29—2003《食品卫生微生物学检验椰毒假单胞菌酵米面亚种检验》进行。米酵菌酸检测按照GB/T 11675—2003《银耳卫生标准》执行,采用液相色谱-质谱联用法对样品开展检测。结果经VITEK 2 COMPACT全自动微生物生化鉴定仪和基因指纹鉴定仪进行鉴定,4份样品中3份鉴定结果为唐菖蒲伯克霍尔德菌,小鼠毒性试验阳性。4份样品均检测出米酵菌酸。结论本次食物中毒源于食源性椰毒假单胞菌酵米面亚种污染。更多还原

【Abstract】 Objective To investigate the pathogen of a food poisoning accident and provide scientific evidence to identifythe cause. Methods Isolation,identification and toxicological assessment of toxic agents were carried out according to the national standard GB / T 4789. 29-2003, bongkrekic acid was detected by liquid-plasma chromatography techniques according to GB / T 11675-2003. Results By VITEK 2 COMPACTR and Ribo-Printer microbial characterization system,Burkholderia gladioli was identified in 3 of 4 food samples,but all of the 4 food samples were confirmed to be contaminated by Pseudomonas cocovenenans subsp. Farino. A positive toxicological result was found in mice acute oral toxicity test.Conclusion The food poisoning accident was associated with ingestion of foods contaminated by Pseudomonas cocovenenans subsp. Farino.更多还原