【摘要】 目的:建立通脉颗粒的质量标准。方法:采用薄层色谱法对方中黄芪、红参、三七、赤芍进行定性鉴别;采用HPLC测定通脉颗粒中三七皂苷R1,人参皂苷Rg1,人参皂苷Re及人参皂苷Rb1的总量。结果:本品TLC定性鉴别薄层色谱斑点清晰,专属性强,重复性好。HPLC测定三七皂苷R1,人参皂苷Rg1,人参皂苷Re及人参皂苷Rb1进样量分别在0.058 8~0.588 0,0.487 9~4.879,0.104 9~1.049,0.308 3~3.083μg呈良好的线性关系,r分别为0.999 6,0.999 6,0.999 7,0.999 6,平均加样回收率为95.34%(RSD 1.8%),95.42%(RSD 2.0%),98.99%(RSD 1.8%),102.13%(RSD 1.8%)。结论:该方法准确灵敏,简便,专属性强、重复性好,适用于通脉颗粒的质量控制。更多还原

【Abstract】 Objective: To establish quality standards of Tongmai granules. Method: Ginseng Radix et Rhizoma Rubra,Astragali Radix,Notoginseng Radix et Rhizoma and Paeoniae Radix Rubra in Tongmai granules were identified by TLC; the contents of notoginsenoside R1,ginseng saponins Rg1,ginseng saponins Re and ginseng saponins Rb1 were determined by HPLC. Result: TLC spots were clear and specific in good repeatability.Linear ranges of notoginsenoside R1,ginseng saponins Rg1,ginseng saponins Re and ginseng saponins Rb1were0. 058 8-0. 588 8,0. 487 9-4. 879,0. 104 9-1. 049 and 0. 308 3-3. 083 μg, average recoveries were 95. 34%( RSD 1. 8%),95. 42%( RSD 2. 0%),98. 99%( RSD 1. 8%) and 102. 13%( RSD 1. 8%),respectively.Conclusion: This method is accurate,sensitive,simple and specific in good repeatability,which is suitable for quality control of Tongmai granules.更多还原