【摘要】 目的:研究刺五加根有效组分在不同质量浓度下的体外抗氧化活性,筛选最佳有效组分,为刺五加根的研究和开发提供参考。方法:采用紫外分光光度法测定各组分中总黄酮和总皂苷含量,检测波长分别为510,550 nm。利用超氧阴离子自由基（O-2·）清除法,羟基自由基（·OH）清除法,1,1-二苯基-2-苦基肼基（DPPH）自由基清除法和亚铁还原能力实验（FRAP）法测定刺五加根不同有效组分的抗氧化活性。结果:不同有效组分的刺五加根在一定质量浓度范围内均有一定的抗氧化活性,且随着质量浓度的增加抗氧化能力越强。在O-2·清除法模型中,质量浓度为20 g·L^-1时,30%乙醇洗脱部位清除率（91.61±2.59）%。在·OH清除法模型中,质量浓度为1.0 g·L^-1时,30%乙醇洗脱部位清除率（90.29±2.06）%。在DPPH自由基清除法模型中,质量浓度为0.7 g·L^-1时,30%乙醇洗脱部位清除率清除率达（84.45±0.14）%。在FRAP法中,质量浓度为1.0 g·L^-1时,30%乙醇洗脱部位的FRAP值3 474±36.84。结论:刺五加根各有效组分均有一定的抗氧化活性,30%乙醇洗脱部位的抗氧化能力最强,可开发成天然的植物抗氧化剂。更多还原

【Abstract】 Objective: To study on antioxidant activity of effective components in Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis roots. Method: Contents of total saponins and total flavonoids were determined by UV. Antioxidant activity of effective components in Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis roots were conducted with superoxide anion free radical scavenging method,hydroxyl free radical scavenging method,DPPH scavenging method and FRAP method. Result: Different effective components in a certain concentration range had some antioxidant activities. When the concentration increased, antioxidant ability enhanced. Clearance of 30% ethanol eluted site on O₂^-· was（ 91. 61 ± 2. 59） % at the concentration of 20 g·L^-1. Clearance of 30% ethanol eluted site on ·OH was（ 90. 29 ± 2. 06） % at the concentration of 1. 0 g·L^-1.Clearance of 30% ethanol eluted site on DPPH was（ 84. 45 ± 0. 14） % at the concentration of 0. 7 g·L^-1. FRAP value of 30% ethanol eluted site was 3 474 ± 36. 84 at the concentration of 1. 0 g·L^-1. Conclusion: All the effective components of Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis roots have a certain antioxidant activity. Antioxidant activity of 30% ethanol eluted site is the highest.更多还原