【摘要】 目的:观察冠心止痛胶囊(GXZT)对动脉粥样化硬化(AS)模型大鼠的影响。方法:SD大鼠随机分为正常组、模型组、冠心止痛胶囊低、高剂量组和阿乐组。正常组动物摄食正常颗粒饲料,模型组及GXZT各组在摄食高脂饲料的基础上采用ip维生素D3(VD3)加激发免疫反应的方法复制AS模型。各组动物连续灌胃90 d,检测血清总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C),Ca2+,超氧化物歧化酶(SOD),丙二醛(MDA),一氧化氮(NO),一氧化氧合酶(NOS),内皮素(ET),白细胞介素-6(IL-6),肿瘤坏死因子α(TNF-α),IL-18,脂联素(ADP),可溶性CD40L(s CD40L),计算动脉硬化指数(AI)和脏器指数;取胸主动脉HE染色,进行病理组织学观察。结果:与正常组比较,模型组大鼠肝脏指数,AI,血清TC,LDL-C,Ca2+,MDA,ET,TNF-α,IL-18,s CD40L均显著升高(P<0.05),而SOD,NO,NOS,ADP均有不同程度降低。与模型组比较,GXZT低、高剂量组大鼠肝脏指数,AI,血清TC,LDL-C均不同程度降低(P<0.05),血清NO,NOS水平均有显著升高(P<0.05);GXZT高剂量组可显著降低血清ET,TNF-α,IL-6,IL-18,s CD40L水平(P<0.05)。HE染色显示模型组主动脉可见内皮损伤,粥样斑块形成,给药各组均可显著抑制其主动脉损伤。结论:GXZT通过改善AS模型大鼠脂质代谢、减少炎性细胞因子的分泌或释放,以及调节血管活性物质等功效,对大鼠AS的形成具有多靶点干预作用,对血清SOD,MDA水平无明显影响。更多还原

【Abstract】 Objective: To investigate the effect of Guanxin Zhitong capsule( GXZT) in atherosclerosis( AS) model rats. Method: SD rats were randomly divided into 5 groups: normal group,model control group,GXZT high and low dose groups and Atorvastatin Calcium tablets control group. The normal group received normal pellet feed. Model group and GXZT groups were injected with Vitamin D3( VD3) based on high-fat fedding and stimulated immune response method was used to establish AS models. Each group was given the corresponding drugs for 90 days. Blood serum were analyzed for total cholesterol( TC),triglyceride( TG),low density lipoprotein cholesterol( LDL-C) and high density lipoprotein cholesterol( HDL-C),calcium( Ca2 +),superoxide dismutase( SOD),malondialdehyde( MDA),nitric oxide( NO),nitric oxide synthase( NOS), levels of endothelin( ET),interleukin-6( IL-6),tumor necrosisfactor-α( TNF-α),interleukin-18( IL-18),adiponectin( ADP),and soluble CD40L( s CD40L). Visceral index and arteriosclerosis index( AI) were calculated; the pathological observation of aorta was carried out by HE staining. Result: The liver index,AI,TC,LDL-C,Ca2 +,MDA,ET,TNF-α,IL-18 and s CD40 L were significantly higher( P < 0. 05) while the SOD,NO,NOS and ADP levels were lower in model group than those in normal group. Compared with those in model group,the liver index,AI,TC,and LDL-C in GXZT low and high dose groups were lower( P < 0. 05),while the serum NO and NOS levels were significantly increased( P < 0. 05). GXZT high dose group could significantly reduce serum ET,TNF-α,IL-6, IL-18 and s CD40 L levels( P < 0. 05). HE staining showed aortic endothelial injury and atherosclerotic plaque formation in model group,and various treatment groups can significantly inhibit aortic lesions. Conclusion: GXZT has intervention effect on atherosclerotic plaque formation of model animals at multiple targets by improving metabolism of lipid in model rats,reducing the secretion or release of inflammatory cytokines,and regulating vascular active substances,but it has no significant effect on serum SOD and MDA levels.更多还原