【摘要】 目的:研究戊己丸药味配伍的特征图谱及其化学成分的变化规律。方法:采用毛细管电泳(HPCE)与反相高效液相色谱法对不同拆方进行考察,毛细管电泳以50 mmol·L-1的硼砂缓冲液-甲醇(2∶1)缓冲液为背景电解质,工作电压25k V,压力进样5 k Pa×15 s,电解质封口5 k Pa×10 s,柱温15℃。反相高效液相色谱法采用Agelapromosil C18色谱柱(4.6 mm×250 mm,5μm),柱温25℃,流速0.8 m L·min-1,流动相乙腈(A)-0.05 mol·L-1磷酸二氢钾(含0.1%磷酸)(B)进行梯度洗脱,检测波长230 nm,进样量5μL。采用撤药分析法对戊己丸进行研究,从定性定量两个角度进行评价,从整体上考察不同配伍情况方剂中化学成分的变化。结果:HPCE法确定了9个特征指纹峰,HPLC法确定27个特征指纹峰,其中黄连为该方君药,其生物碱类成分在不同配伍中变化较大;白芍中成分变化较小。结论:运用特征图谱技术结合定性、定量方法研究复方配伍,对复方新剂型的研制起到一定的指导作用。更多还原

【Abstract】 Objective: To study the characteristic chromatogram of herbs in Wuji Wan and the change rules of the chemical components. Method: HPLC and HPCE method were used to study different separated prescriptions. The conditions for the capillary zone electrophoresis( CZE) method were 50 mmol·L- 1Borax bufferMethanol( 2∶ 1),constant voltage of 25 k V,pressure sampling under 5 k Pa × 15 s,electrolyte sealing under5 k Pa × 10 s and column temperature at 15 ℃. Chromatographic experiments were performed on Agelapromosil C18column( 4. 6 mm × 250 mm,5 μm),with the mobile phase was acetomitrile( A)-0. 05 mol·L- 1potassium dihydrogen phosphate( including 0. 1% phosphoric acid)( B) by a gradient elution; the detection wavelength was230 nm,column temperature was 25 ℃,the flow rate was 0. 8 m L·min- 1. The withdrawal analytical method was used for a qualitative and quantitative evaluation on Wuji Wan,in order to study the changes in chemical components in different prescriptions. Result: HPCE and HPLC were used to confirm 9 and 27 characteristic fingerprint peaks. Coptidis Rhizoma was the main herb of the prescriptions,with great changes in alkaloids in different prescriptions and small changes in Paeoniae Alba Radix. Conclusion: The fingerprint technology and the qualitative and quantitative methods are combined to guide the development of new compound prescriptions.更多还原