【摘要】 对植物乳杆菌(Lactobacillus plantarum)SD2、发酵乳杆菌(Lactobacillus fermentum)SD4在5 L立瓶中分别进行培养,制备尾菜液体青贮菌剂。结果表明,在MRS液体培养基中37℃条件下,调控pH值,菌株SD2和SD4最适收获期分别为24 h、20 h,菌株SD2与SD4配比为2∶1时青贮效果最佳,尾菜液体青贮菌剂总活菌数>1×10~9 CFU/mL。按0.5%接种量,进行废弃白菜叶、青笋叶青贮发酵应用试验,生产的尾菜青贮饲料品质良好,颜色淡黄色或黄绿色,酸香味浓郁,质地较松散,pH值<4.0。研制的尾菜青贮菌剂适用于尾菜青贮。更多还原

【Abstract】 By the cultivation of Lactobacillus plantarum SD2 and Lactobacillus fermentum SD4 in 5 L roller bottle, the bacterial inoculants for vegetable wastes silage were prepared. The results showed that in MRS liquid medium, at fermentation temperature 30 ℃, by adjusting p H value, the optimum harvesting time of L. plantarum SD2 and L. fermentum SD4 were 24 h and 20 h, respectively. Under the condition of L. plantarum SD2 and L. fermentum SD4 ratio 2∶1, the silage effect was the optimum, and the total viable count of bacterial inoculants for vegetable wastes silage was more than 1×10~9CFU/ml. The waste Chinese cabbage leaves and asparagus leaves were fermented by silage bacteria inoculants with inoculum 0.5%, and the vegetable wastes silage had good quality, light yellow or yellowish green color, strong acid fragrance and friable texture. The both vegetable wastes silage p Hs were less than 4.0. The silage bacteria inoculants developed were suitable for the vegetable wastes silage.更多还原