【摘要】 目的采用雨蛙肽、L-精氨酸、牛磺胆酸钠作为诱导剂建立大鼠急性胰腺炎（AP）动物模型,通过大鼠病理评分、血清生化及炎症细胞因子指标检测,找出一种操作简单、重复性好的大鼠AP造模方法。方法采用不用造模方法处理后的大鼠在不同时间点采集血液及胰腺标本。检测指标有淀粉酶（AMY）、谷丙转氨酶（ALT）、尿素氮（BUN）、肌酐（Cr）、Ca²⁺、超氧化物歧化酶（SOD）、白介素（IL）-6、IL-10、肿瘤坏死因子（TNF）-α及胰腺组织病理评分。结果雨蛙肽组大鼠血清检查结果及胰腺病理均符合轻型AP改变,L-精氨酸及牛磺胆酸钠组血清检查结果及胰腺病理均符合重症AP改变。结论三种方法均可成模,大鼠AP模型病情最重的是牛磺胆酸钠诱导组,其次为L-精氨酸诱导组,病情最轻为雨蛙肽诱导组。更多还原

【Abstract】 Objective To find out a simple and reproducible methods of acute pancreatitis( AP) model rat.Methods Rats were received different methods to set up AP rat models.Serum biochemical indicators and pancreas specimens of the four groups rats were tested after AP induction.The levels of AMY,ALT,BUN,Cr,Ca2+,SOD,IL-6,IL-10,TNF-α were detected,and pathological scores of pancreas tissues were measured.Results Serum indicators and pathological score of caerulein group were in line with mild AP,yet those of L-arginine and sodium taurocholate group were in line with severe AP. Conclusions The models of AP rat could be established successfully by the three ways,the model induced by sodium taurocholate was the most serious illness and the caerulein was the lightest condition according to the three experimental groups at the same time point.更多还原