【摘要】 目的探讨1,8-桉油素预处理对Aβ_{25 35}诱导原代培养大鼠皮层神经元炎症反应的影响。方法原代培养大鼠皮层神经元,随机分组。采用噻唑蓝（MTT）还原反应观察神经元损伤;以Aβ_{25 35}处理24 h诱导神经元损伤;酶联免疫吸附法（ELISA）检测上清液中肿瘤坏死因子（TNF）-α、白介素（IL）-1β及半胱氨酰白三烯（Cys LTs）释放水平。结果 20μmol/L的Aβ_{25 35}处理神经元细胞24 h可显著降低神经元活性,增加TNF-α、IL-1β及Cys LTs的释放,1,8-桉油素10μmol/L预处理后可明显抑制Aβ_{25 35}引起的细胞毒性效应,并抑制上述炎症因子及Cys LTs的释放。结论 1,8-桉油素对Aβ_{25 35}诱导的神经元损伤具有保护作用,与抑制TNF-α、IL-1β及Cys LTs的释放有关。更多还原

【Abstract】 Objective To investigate the effect of 1,8-cineol against Aβ_{25 35}-induced inflammatory response in rat primary cultured neurons. Methods Rat primary cultured cortical neurons were randomly divided into control,Aβ_{25 35}（ 20 μmol/L）,low dose of 1,8-cineol( Aβ_{25 35}20 μmol / L + 1,8-cineol 1 μmol / L),high dose of 1,8-cineol( Aβ_{25 35}20 μmol / L +1,8-cineol 10 μmol / L) groups. Cell viability was evaluated by 3-（ 4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide（ MTT） reduction assay. Neuron injury was induced24 h after Aβ_{25 35} treatment. Releases of TNF-α,IL-1β and Cys LTs in the supernatant were determined by ELISA. Results 20 μmol / L of Aβ_{25 35} incubated for 24 h significantly reduced neuronal activity,and increased TNF-α,IL-1β and Cys LTs releases. Pretreatment with 1,8-cineol obviously inhibited cytotoxicity and decreased the level of above cytokines and Cys LTs releases. Conclusions 1,8-cineol has a protective effect on Aβ_{25 35} induced neuron injury,probably relates with inhibition of TNF-α,IL-1β and Cys LTs releases.更多还原