【摘要】 目的:探讨自体肿瘤抗原负载树突状细胞联合细胞因子诱导的杀伤细胞(DC-CIK)治疗胃癌的疗效及安全性。方法:回顾性分析2005年1月至2010年1月在福建省肿瘤医院行胃癌切除术患者270例,根据是否进行DC-CIK治疗,分为对照组(150例)和DC-CIK治疗组(120例)。应用流式细胞术分析DC-CIK细胞表型,比较两组患者的总生存期(OS),观察DC-CIK治疗的不良反应,Cox回归法分析胃癌预后影响因素。结果:成熟DC中HLA-DR~+、CD8~+、CD83~+和CD86~+的细胞比例分别为(96.16±1.51)%、(96.58±2.66)%、(96.44±2.20)%和(98.74±0.76)%。CIK中CD3~+、CD3~+CD4~+、CD3~+CD8~+和CD3~+CD16~+/CD56~+的细胞比例分别为(91.98±5.9)%、(25.19±10.5)%、(71.15±7.8)%和(18.73±7.4)%。DC-CIK治疗组中位OS为77个月,与对照组的51个月相比差异明显(χ2=4.431,P=0.035)。DC-CIK治疗、辅助化疗和TNM分期是胃癌预后的独立影响因素。DC-CIK治疗的常见不良反应为发热、寒战和乏力。结论:胃癌术后应用自体肿瘤抗原负载DC-CIK治疗可延长胃癌患者的OS,且不良反应轻,安全有效。更多还原

【Abstract】 Objective: To explore the curative effect and safety of autologous tumor antigen loaded dendritic cells combined with cytokine-induced killer( DC-CIK) cells in the treatment of patients with gastric cancer. Methods: Two hundred seventy patients who received operative resection of gastric cancer in Fujian Provincial Cancer Hospital during January,2005 to January,2010 were retrospectively analyzed. Acording to wether receiving DC-CIK therapy,the patients were divided into DC-CIK treatment group(120 cases) and control group(150 cases). Flow cytometry assay was used to analyze phenotype of DC-CIK cells. Overall survival( OS) between both groups was compared. Advers reactions of the DC-CIK therapy were observed. And prognostic factors of the gastric cancer were analyzed by Cox regression model.Results: In the mature DC,the percentages of HLA-DR~+,CD8~+,CD83~+and CD86~+cells were( 96. 16 ± 1. 51) %,(96. 58 ± 2. 66)%,(96. 44 ± 2. 20)%,and(98. 74 ± 0. 76)% respectively. In CIK cells. the proportions of CD3~+,CD3~+CD4~+,CD3~+CD8~+and CD3~+CD16~+/ CD56~+cells were(91. 98 ± 5. 9) %,(25. 19 ± 10. 5) %,(71. 15 ± 7. 8) %and(18. 73 ± 7. 4) % respectively. The median OS was 77 months in the DC-CIK treatment group and compared to OS median of 51 months in the control group,the difference was significant( χ2= 4. 431,P < 0. 05). DC-CIK therapy,adjuvant chemotherapy and TNM stage are independent prognostic factors for gastric cancer. Common adverse reactions are fever,chill and weakness in the DC-CIK therapy. Conclusion: Autologous tumor antigen-loaded DC-CIK therapy could prolong OS of patients with gastric cancer after operation of gastric cancer,and have little adverse reactions as well as be safety and effective.更多还原