目的:探讨热激胃癌细胞来源外泌体致敏树突状细胞(dendritic cells,DCs)诱导的肿瘤特异性细胞毒T细胞(cytotoxic T lymphocyte,CTL)反应。方法:制备小鼠前胃癌细胞(murine foregastric cancer cells,MFC)来源外泌体(Exo)、热激MFC细胞来源外泌体(Exo/HS)及MFC细胞冻融抗原(lysates,Lys),通过电镜观察外泌体的形态,Western blotting检测外泌体的成分。培养小鼠骨髓来源的DCs,将Exo/HS、Exo和Lys冲击致敏DCs,制备的DCs瘤苗分别命名为DC-Exo/HS、DC-Exo和DC-Lys。HSP70小干扰RNA(small interference RNA,siRNA)转染MFC细胞,热激后分离上清,用制备外泌体致敏DCs,流式术检测DCs的表型。以DC-Exo/HS、DC-Exo、DC-Lys、DC和PBS免疫小鼠,~3H-TdR法检测脾脏T细胞的增殖,LDH法检测脾细胞CTL活性。建立MFC细胞荷瘤小鼠模型,观察各组DCs瘤苗的免疫治疗效应。结果:电镜确认外泌体为膜性小囊泡。Western b...
【英文摘要】
Objective: To explore response of tumor-specific cytotoxic T lymphocyte( CTL) induced by dendritic cells( DCs) sensitized with exosomes that derived from heat-shocked gastric cancer cell. Methods: exosomes from mice murine foregastric cancer( MFC) cells( Exo),heat-shocked MFC cells( Exo / HS),and lysates( Lys) of MFC cells were prepared,respectively. Morphology of the exosomes was observed by electron microscopy and protein components of the exosomes were detected by Western blotting. DCs generated from mur...
【基金】
浙江省自然科学基金资助项目(No.LY14HY160011)~~
【更新日期】
2016-04-18
【分类号】
R735.2
【正文快照】
杭州310058)[Chin J Cancer Biother,2016,23(2):188-194.DOI:10.3872/j.issn.1007-385X.2016.02.006]树突状细胞(dendritic cells,DCs)是唯一能够活化初始T细胞并启动早期免疫反应的一类抗原提呈细胞,是免疫反应的始动者,其通过独特的摄取、加工并向T细胞提呈抗原的能力调控免
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