Objective: To observe the effect of alcohol derivatives and some non-ionic surfactants on Trinder reaction. Methods: Using the aqueous solution of alcohol derivatives and non-ionic surfactant as sample,Trinder reaction experiments were carried out. The color changes were observed with the naked eye,and the absorbance values were recorded using the automatic biochemical analyzer. Effects of nonionic surfactants on the detection of uric acid were also observed. Results: The results of Trinder reaction experim...