【摘要】 目的:探索微RNA(miRNA,miR-)-let-7e-3p在不同宫颈病变中表达情况及临床意义。方法:收集并整理具有完整临床病理资料的病例,其中正常子宫颈26例、子宫颈高级别鳞状上皮内病变(HSIL)37例、宫颈癌101例;采用实时定量RT-PCR检测临床宫颈组织样本中miR-let-7e-3p的相对表达量,分析miR-let-7e-3p表达水平与宫颈癌的发生及临床不良预后因素间的相关性。将miR-let-7e-3p模拟物转染至宫颈癌细胞株Siha(转染组),采用流式细胞仪检测Siha细胞周期和细胞凋亡变化;CCK-8试剂盒检测Siha细胞增殖变化;Transwell实验检测Siha细胞迁移和侵袭能力变化。结果:miR-let-7e-3p在正常宫颈、HSIL及宫颈癌组织中的相对表达量分别为1.45±0.24、0.79±0.05、0.46±0.04,正常子宫颈与HSIL、正常子宫颈与宫颈癌、HSIL与宫颈癌相对表达量差异均具有统计学意义(均P<0.05);miR-let-7e-3p表达水平与患者宫颈癌临床不良预后因素之间无显著相关性;转染组S期细胞(29.76%±6.60%)高于阴性对照组(13.38%±1.30%,P<0.05);转染组凋亡率(5.98%±1.38%)高于阴性对照组(3.53%±0.79%,P<0.05);转染组转染后48、72、96 h吸光度值分别为0.57±0.11、0.65±0.04、0.84±0.14,阴性对照组为0.74±0.05、0.93±0.10、1.47±0.14,细胞增殖均被抑制(均P<0.05);转染组与阴性对照组迁移和侵袭能力差异均无统计学意义(均P>0.05)。结论:miR-1et-7e-3p可能在宫颈癌的发生中发挥抑癌基因的作用,阻滞细胞周期和抑制细胞增殖可能是其重要调控机制。更多还原

【Abstract】 Objective:To investigate the expression of microRNA(miRNA,miR)let-7e-3p in different cervical lesions and its clinical significance.Methods:The expression of miR-let-7e-3p in the tissues of normal cervix(n = 26),high-grade squamous intraepithelial lesion(HSIL)(n = 37),and cervix carcinoma(n = 101)were detected by reverse transcription and quantitative polymerase chain reaction(RT-qPCR).The correlation of miR-let-7e-3p expression with the clinicopathological parameters of patients with cervical cancer was analyzed.miR-let-7e-3p mimic was transfected into cervical carcinoma Siha cells.The cell cycle and apoptosis were determined by flow cytometry;cell proliferation was determined by CCK-8 kit;and the migration and invasion of cells were determined by Transwell assay.Results:The relative expression levels of miR-let-7e-3p in normal cervix,HSIL,and cervical carcinoma were 1.45 ± 0.24,0.79 ± 0.05 and 0.46 ± 0.04,respectively(all P <0.05).After transfection with miR-let-7e-3p mimic,die S-phase fraction and apoptosis rate of Siha cells were increased significantly compared with control group[(29.76 ±6.6)%vs(13.38 ±1.3)%,P<0.05;(5.98 ±1.38)%vs(3.53 ±0.79)%,P<0.05,respectively].OD of transfected Siha cells at 48,72 and 96 h were 0.57 ±0.11,0.65 ±0.04 and 0.84 ±0.14,which were significantly lower than those of untransfected Siha cells(0.74 ±0.05,0.93 ±0.10 and 1.47 ±0.14,all P <0.05).The migration and invasion abilities of transfected Siha cells were not significantly changed(all P >0.05).Conclusion:The expression of miR-let-7e-3p is down-regulated in cervical neoplasms,which is associated with cell cycle arrest and proliferation inhibition of cervical cancer cells.更多还原