【摘要】 [目的]制备一种掺锶硫酸钙与DBM复合的骨修复材料,为临床提供一种成骨活性优良的骨修复材料。[方法]制备掺锶硫酸钙与DBM复合的活性骨修复材料,其中锶元素的量为钙含量的6%,掺锶硫酸钙与DBM按体积比1∶1复合。观察兔股骨髁骨缺损模型修复效果,利用X线片观察缺损区域的新骨形成情况,采用MicroCT评价局部的骨量和骨质变化,对组织切片予以Van Gieson（VG）复合染色法染色进行组织形态学观察,荧光双标观测计算成骨速率。[结果]术后动物无死亡,所有兔子切口无渗血或出现分泌物状况,术后活动正常。从MicroCT的BV/TV结果看出实验组在12周时缺损区域已经恢复;掺锶CaSO₄复合材料组缺损部位已经基本修复,CaSO₄组未完全修复。VG组织学观察显示各组均有新生骨小梁生成,荧光双标观测得到的矿化沉积率显示复合材料组新骨生长突出,矿化沉积率为（2.31±0.13）μm/d。[结论]通过MicroCT、VG组织学染色以及荧光双标观测对兔子股骨髁缺损修复效果进行评价,发现复合材料具有较好的骨缺损修复能力。更多还原

【Abstract】 [Objective]To investigate the bone repairing effect of an composited material consisted of strontium-doped calcium sulphate and DBM in a rabbit model. [Method]The composite material consisted of strontium-doped calcium sulphate and DBM was prepared. The amount of strontium was 6% compared to the calcium,and strontium-doped calcium sulphate and DBM were composited by volume ratio of 1: 1. The bilateral femoral condyle of 12 rabbits was used as defect models. Animals were then divided into CaSO₄ sub-control group,CaSO₄+SrCO₃ sub-control group,and CaSO₄+ SrCO₃+ DBM group（ the experiment group）,with 8 defects in each group. The changes of bone density and local bone mass were evaluated by X-ray and Micro-CT,respectively. Histological observation was carried out with Van Gieson dyeing method. In addition,the mineralization rate was observed after fluorescence labeling. [Result]There was no death of the animals after surgery. Based on the results of X-ray and Micro-CT,the defect of composited material group（ the experiment group） had been recovered at 12 week,while it was basically repaird in CaSO₄+ SrCO₃ sub-control group and not completely repaired in CaSO₄ sub-control group.The histological observations of VG indicated new bone trabecula growing in each group,and no inorganic powder and DBM were found. The result of histological observation was consistent with that of X-ray and Micro-CT. Yellow green marks could be seen in all the samples under the fluorescence microscope,the rate of mineralization in composited material group was the highest,reaching 2. 31 ± 0. 13 μm / d. [Conclusion]The composited material has high osteogenic capacity.更多还原