【摘要】 人C-反应蛋白(C-reactive protein,CRP)参与人体炎症反应,是临床诊断中的重要检测指标。利用家蚕杆状病毒表达系统构建重组人CRP病毒vBmHis-CRP,接种家蚕五龄幼虫表达重组人CRP蛋白。利用自制抗CRP血清Western Blot检测重组蛋白得到目的条带,纯化后BCA法测定蛋白含量为30μg/mL,质谱检测进一步确定该蛋白为目的蛋白。这为家蚕生物反应器规模化制备重组人CRP蛋白打下了基础,为解决临床CRP相关炎症反应快速诊断试剂盒中标准抗原的来源问题提供了依据。更多还原

【Abstract】 C-reactive protein(CRP),which involves in human inflammatory response,is an important clinical diagnosis index.In this study,we used silkworm baculovirus expression system to construct recombinant vBmHis-CRPvirus,which was then inoculated to fifth instar silkworm express recombinant CRP protein.The target band was obtained by using self-made CRP resistance Western Blot to detect recombinant protein.The content of the purified protein measured by BCA method was 30μg/mL,and the target protein was further identified through mass spectrometric detection.The present work laid a foundation for the large-scale preparation of recombinant CRP protein by silkworm bioreactor,and provided a basis for solving the source problem of standard antigens in the clinical CRP-related inflammatory response rapid diagnostic kits.更多还原