Objective To investigate the killing effect of hypericin on tachyzoites of Toxoplasma gondii RH strain in vitro. Methods Normal saline( group A) and different concentrations of hypericin( 5 μg/ ml, group B;50 μg / ml, group C; 500 μg/ml, group D) were added to T. gondii tachyzoites in 24-well plate(1 ×10~6 /well). The tachyzoites were harvested after 2, 4 and 6 h, and underwent the following treatment: trypan blue staining to calculate the dyeing rate, Giemsa staining to observe the morphological and struct...
(1 Department of Microbiology and Parasitology,Anhui Key Laboratory of Infection and Immunity,Bengbu Medical College,Bengbu 233030,China;2 Department of Parasitology,School of Basic MedicalSciences,Capital Medical University,Beijing 100069,China;3 Departm