【摘要】 根据临床和实验室标准协会(clinical and laboratory standards institute,CLSI)推荐的微量肉汤稀释法检测泥样中大肠杆菌对四环素的敏感性,并应用PCR法检测四环素耐药基因tet A,tet B,探讨氮磷营养盐对大肠杆菌四环素耐药性影响的可能机制。结果表明:添加不同剂量氮磷营养盐能够导致大肠杆菌对四环素产生耐药性,但大肠杆菌的耐药率与剂量间无明显相关性。37株高耐药率大肠杆菌tet A基因的阳性率为100%,66株低耐药率大肠杆菌tet A基因的阳性率为16.67%,而16株对药物敏感的大肠杆菌未检测到tet A基因;但供试大肠杆菌均未检测到tet B基因。这表明,氮磷营养盐诱导的大肠杆菌四环素耐药性与tet A基因有关。更多还原

【Abstract】 In the present study,sensitivity of Escherichia coli strains to tetracycline( TE) was detected by broth micro-dilution method,and tet A,tet B genes of E. coli strains were detected by PCR methods to explore the possible mechanisms. It was shown that addition of different concentrations of nitrogen and phosphorous induced resistance of E. coli to TE. But,there was no significant correction between TE resistance of E. coli and nitrogen or phosphorus dose. For 37 strains with high TE resistance,the detection rate of tet A gene was 100%; for 66 strains with lower TE resistance,the detection rate of tet A gene was 16. 67%; and no tet A gene was detected in the strains sensitive to TE.Interestingly,no tet B gene was detected in either TE resistant or sensitive E. coli strains,which indicated that tet A gene,other than tet B gene,might be involved in the nitrogen and phosphorus induced TE resistance of E. coli.更多还原