【摘要】 目的 探讨茶多酚(TP)对心肺复苏后大鼠脑组织过氧化物歧化酶(SOD)表达及细胞凋亡的影响。方法 经食道交流电刺激诱导大鼠发生心室颤动(VF),建立心脏骤停/心肺复苏(CA/CPR)模型。假手术(SH)组只行手术操作,不诱导CACF·R(n=6)。恢复自主循环的48只大鼠随机分成两组,分别为TP组(10mg/kg,iv.,n=24)和模型组(注射等量生理盐水,n=24),每组分别设12 h、24 h、48 h、72 h四个检测时间点,每个时间点有6只大鼠。用免疫荧光标记法检测大脑皮层SOD的荧光密度,Caspase3/TUNEL双标记法检测细胞凋亡。结果 CPR后,模型组和TP组在12 h、24 h、48 h的SOD荧光密度均较假手术组明显降低(P<0.05);TP组在四个时间点的SOD荧光密度均较模型组明显升高(P<0.05)。CPR后72 h,TP组的Caspase3/TUNEL阳性率较模型组明显降低(P<0.05)。结论 茶多酚能提高心脏骤停/心肺复苏后大鼠脑组织SOD的表达水平,抑制细胞凋亡。更多还原

【Abstract】 Objective This essay is written to explore whether tea polyphenols(TP) improves the expression of superoxide dimutase and inhibits cell apoptosis in brain tissue after cardiopulmonary resuscitation(CPR).Methods Cardiac arrest/cardiopulmonary resuscitation(CA/CPR) model was established using the method of alternating current-esophagus stimulation.Sham group(SH) was treated without CA/CPR(n= 6).Forty-eight rats,once spontaneous circulation restoring,were divided into two groups,tea TP group(10 mg/kg,iv.,n= 24) and model group(injection equal of normal saline,n= 24).Each group respectively was set 12 h,24 h,48 h,72 h four detection points,with 6 rats in each time point.The cerebral tissue of superoxide dismutase fluorescence density and apoptotic cells were detected with immunofluorescence labeling method and Caspase3/TUNEL double labeling method.Results After CPR,the SOD fluorescence density of both model and TP group was lower than that of control group(P<0.05).The density in TP group was significantly higher than that in the model group at four time points(P< 0.05).72 h after CPR,Caspase3/TUNEL positive rate of TP group was obviously lower in the model group(P < 0.05).Conclusion Tea polyphenol can improve the SOD expression and inhibit cell apoptosis in brain tissues of rats after CPR.更多还原