【摘要】 目的探讨血管内皮生长因子(VEGF)、p53蛋白在甲状腺肿瘤中的表达及对鉴别诊断甲状腺良恶性肿瘤的价值。方法纳入2011年10月至2015年10月本院收治的103例甲状腺肿瘤患者,均行手术治疗,收集病理标本,利用免疫组化分析技术检测其VEGF及p53蛋白的表达。以术后病理诊断为金标准,探讨VEGF及p53蛋白对鉴别甲状腺恶性肿瘤的价值。结果 1VEGF及p53在甲状腺恶性肿瘤中阳性表达率均明显高于良性肿瘤中,差异有显著性意义(P<0.05),且在乳头状癌、滤泡癌、未分化癌中阳性率均逐渐上升;2在所有标本中,VEGF与p53蛋白的表达均呈明显正相关(r=0.685,P<0.05);3VEGF对甲状腺恶性肿瘤的诊断敏感度0.638、特异度0.889、准确率74.8%;p53对甲状腺恶性肿瘤的诊断敏感度0.466、特异度0.822、准确率62.1%。结论 VEGF及p53蛋白对鉴别甲状腺良恶性肿瘤、初步判断肿瘤组织学类型有一定价值,p53蛋白可上调VEGF表达。更多还原

【Abstract】 Objective To investigate the expression of vascular endothelial growth factor(VEGF) and p53 protein in thyroid tumors and to evaluate the value of differential diagnosis of benign and malignant tumors of the thyroid gland. Methods from October 2011 to October 2015, 103 patients with thyroid tumor were treated with operation in our hospital were selected. The pathological samples were collected. The expression of VEGF and p53 protein was detected by immunohistochemical technique. The value of VEGF and p53 protein in the differential diagnosis of thyroid malignant tumor was discussed with the postoperative pathological diagnosis as the gold standard. Results The expression rate of VEGF and p53 in thyroid malignant tumor positive was significantly higher than that in benign tumors( P <0.05), and the positive rate were increased gradually in the papillary cancer, follicular carcinoma, undifferentiated carcinoma. In all specimens, the expression of VEGF and p53 protein was significantly positive correlation(r=0.685, P<0.05). The diagnosis sensitivity of VEGF in thyroid malignant tumor was 0.638, specificity was 0.889, accurate rate was 74.8%.The diagnosis sensitivity of p53 in thyroid malignant tumor was 0.466, specificity was 0.822, accurate rate was 62.1%. Conclusion VEGF and p53 protein had certain value in differentiating benign and malignant thyroid tumor, and the type of tumor. P53 protein could regulate the expression of VEGF.更多还原