Objective To clone,express,and identify the RRas gene of Trichomonas vaginalis in order to further study its location and functions in tissues and cells. Methods A cDNA fragment of T.vaginalis RRas was amplified with PCR.In one step,the fragment was cloned into a PQE-80 LEasy vector.Clones with the PQE-80L/T.vaginalis RRas gene were identified with PCR and restriction enzyme digestion and then sequenced.E.coli BL21 cells were transformed with the recombinant plasmid.IPTG was used to induce expression of the...
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