Objectives To study the relationship between deltamethrin resistance and gender in male and female Culex pipiens pallens. Methods A cross of an insecticide-susceptible strain(S)and a deltamethrin-resistant strain(R)of Culex pipiens pallens was generated,and the F2 hybrids were divided into 4phenotypes using the WHO standard susceptibility test.De novo transcriptome sequencing(RNA-seq)was used to analyze the difference in resistance in male and female mosquitoes. Results Results indicated that protein O-GlcN...