Objectives To prepare a soluble form of Schistosoma japonicum enolase and to explore its immunological features and value in immunological diagnosis of schistosomiasis. Methods A cDNA fragment encoding Schistosoma enolase was synthesized using a reverse transcription polymerase chain reaction(RT-PCR),and the fragment was subcloned into the expression plasmid pET28a(+)to construct a recombinant expression plasmid.The recombinant expression plasmid was transformed into E.coli BL21,and expression of soluble re...
***levels were effective at diagnosing schistosomiasis.Results The gene encoding S.japonicumenolase was successfullycloned,and expression of soluble recombinant S.japonicumenolase was induced at a low temperature of 18?C.The pu-rified recombinant protein
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