Objectives To construct a murine toxin deletion mutant of Yersinia pestis and to establish a method of knocking out Y.pestis genes to lay the foundation for the study of the genes of Y.pestis. Methods An attenuated vaccine strain(0614F)was used as the experimental strain.A pair of PCR primers was designed to amplify chloramphenicol resistance genes.The software Artemis was used to read the gene sequences of 91,001 strains to identify toxin genes of Y.pestis with homologous arms.λ-red based mutagenesis was u...
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