【摘要】 [目的]评价别直参对MCF-7细胞体外增殖的影响,并检测其对凋亡相关蛋白bax/bcl-2表达的影响。[方法]采用MTT比色法检测对MCF-7细胞增殖的作用;流式细胞术检测对MCF-7细胞凋亡的影响;免疫组化法检测bax/bcl-2的表达。[结果]别直参高剂量组作用MCF-7细胞24h后,MTT检测增殖率为89.83%,提示有抑制MCF-7细胞体外增殖的作用(P<0.05);流式细胞术检测高剂量组细胞凋亡率明显升高(P<0.05);免疫组化法检测高剂量组能上调MCF-7细胞bax的表达,并下调bcl-2的表达。[结论]别直参高剂量组具有一定的诱导MCF-7细胞凋亡效应作用,其机制可能通过上调bax的表达和下调bcl-2的表达。更多还原

【Abstract】 [Purpose] To investigate the impact of serum containing Korean Ginseng on MCF-7 cell proliferation and the expression of bax/bal-2 proteins. [Methods] By using the MTT method to detect cell proliferation and flow cytometry to examine cell apoptosis,while the bax/bcl-2 proteins were detected by IHC. [Results] The high dose of Korean ginseng could make an anti-proliferative effect on MCF-7 cell with the proliferation rate of 89.83% by MTT method as well as high apoptosis rate by flow cytometry(P<0.05). The IHC revealed high dose of Korean ginseng increase the expression of bax protein and decrease the expression of bcl-2 protein. [Conclusion] The high dose of Korean ginseng induced apoptosis in MCF-7 cell by increasing the expression of bax protein and decreasing the expression of bcl-2 protein.更多还原