【摘要】 该文研究植物蛋白和动物蛋白对气阴两虚2型糖尿病大鼠氨基酸代谢谱的影响。将110只雄性SD大鼠随机分为空白组（10只）,糖尿病模型组（20只）,病症结合组（80只）,正常组大鼠给予普通饲料,其余给予高糖高脂饲料全程喂养,期间病症结合组灌服青皮附子(15.75 g·kg^-1)每日1次,5周后,腹腔注射小剂量链脲佐菌素(40 mg·kg^-1)建立实验性糖尿病大鼠模型,将病症结合成模的大鼠按照血糖随机分为病症结合1组(气阴两虚糖尿病组,15.75 g·kg^-1),病症结合2组(植物蛋白组,0.5 g·kg^-1),病症结合3组(动物蛋白组,0.5 g·kg^-1),病症结合4组(黄连素组,0.1 g·kg^-1),连续灌胃给药4周。蛋白干预4周后,腹主动脉采血分离其血清,运用AQC柱前衍生高效液相色谱法,利用荧光检测器对血清中游离氨基酸进行分析。蛋白干预4周后,植物蛋白、动物蛋白与黄连素对2型糖尿病大鼠体重和血糖无明显影响。植物蛋白组与动物蛋白组相比较,气阴两虚2型糖尿病大鼠血清中组氨酸和脯氨酸（P<0.01）、丝氨酸、甘氨酸、苏氨酸、丙氨酸、酪氨酸、缬氨酸、蛋氨酸、亮+异亮氨酸、苯丙氨酸、赖氨酸（P<0.05）有明显的变化。研究结果显示,蛋白灌胃对气阴两虚2型糖尿病SD大鼠氨基酸代谢产生了影响,通过代谢组学技术找到标志性差异化合物,从而为2型糖尿病早期预警和气阴虚证侯提供实验依据。更多还原

【Abstract】 To study the effect of plant protein and animal protein on amino acid metabolism spectrum of Qi and Yin deficiency type2 diabetic rats. 110 male SD rats were randomly divided into blank group（ n = 10）,diabetic model group（ n = 20）,disease-symptoms group（ n = 80）. The rats of blank group received ordinary feeding,while other groups were fed with high sugar and fat diets. During the whole process of feeding,rats of disease-symptoms group were given with Qingpi-Fuzi( 15. 75 g·kg^-1) once a day through oral administration. Five weeks later,the rats were given with a low dose of STZ( 40 mg·kg^-1) by intraperitoneal injection to establish experimental diabetic models. Then the models were randomly divided into disease-symptoms group 1( Qi and Yin deficiency diabetic group,15. 75 g·kg^-1),disease-symptoms group 2( plant protein group,0. 5 g·kg^-1),disease-symptoms group 3( animal protein group,0. 5 g·kg^-1),disease-symptoms group 4( berberine group,0. 1 g·kg^-1). The drugs were given for 4 weeks by gavage administration. After 4 weeks of protein intervention,the abdominal aortic blood was collected and serum was isolated to analyze its free amino acid by using AQC pre-column derivatization HPLC and fluorescence detector. Four weeks after the protein intervention,plant protein,animal protein and berberine had no obvious effect on body weight and blood sugar in type 2 diabetic rats. As compared with animal protein group,histidine and proline（ P < 0. 01）,serine,glycine,threonine,alanine,tyrosine,valine,methionine,bright +isoleucine,phenylalanine and lysine（ P < 0. 05） changed a lot in rats serum of plant protein group. The results showed that gavage administration of protein would produce effects on amino acid metabolism of Qi and Yin deficiency type 2 diabetic SD rats. Symbolic differential compounds could be found through metabonomics technology,providing experimental basis for early warning of type 2 diabetes and diagnosis of Qi and Yin deficiency syndrome.更多还原