【摘要】 建立了HPLC-ELSD测定醉鱼草果实中三萜类成分含量的方法。采集不同产地醉鱼草果实药材共9批,采用反相高效液相色谱-蒸发光散射检测法测定其三萜成分。色谱条件:Waters Sun FireTMC18柱(4.6 mm×150 mm,5μm),流动相为甲醇-水(82∶18)溶液,体积流量1 m L·min-1,柱温30℃。ELSD参数:漂移管温度106℃,喷雾温度50℃,载气(N2)流速1.5 L·min-1,增益系数1。结果表明醉鱼草果实中的3种三萜成分clinoposaponinⅢ,desrhamnoverbascosaponin,mimengosideⅠ的进样量分别在0.702~28.08μg(r=0.999 2),0.390~15.60μg(r=0.998 9),0.192~7.68μg(r=0.999 0)呈良好线性关系;平均加样回收率(n=6)分别为99.41%,99.08%,98.67%,相对标准偏差分别为0.86%,1.6%,1.8%。该方法简便、准确、可靠,适用于测定醉鱼草果实中三萜类成分的含量。更多还原

【Abstract】 To establish an HPLC-ELSD method for the quantification of triterpenoids in the fruits of Buddleja lindleyana. The RPHPLC-ELSD method was used for the determination of triterpenoids in B. lindleyana fruits,which were collected from different habitats. The column used was a packed with 5 μm stationary phase Waters Sun FireTMC18( 4. 6 mm × 150 mm,5 μm). The mobile phase consisted of Methanol-water( 82∶ 18) at a flow rate of 1 m L·min- 1. Column temperature: 30 ℃. ELSD conditions: drift tube temperature: 106 ℃; carrier gas( nitrogen) flow rate: 1. 5 L·min- 1; amplification factor: 1. The calibration curves showed good linear relationship on a range from 0. 702 to 28. 08 μg( r = 0. 999 2) for Clinoposaponin III,0. 390 to 15. 60 μg( r = 0. 998 9) for Desrhamnoverbascosaponin and 0. 192 to 7. 68μg( r = 0. 999 0) for Mimengoside I. The average recovery rate( n = 6) were 99. 41%,99. 08%and 98. 67% and it’s RSD were 0. 86%,1. 56% and 1. 80%. This method can be used to determine the contents of triterpenoids in the fruits of Buddleja lindleyana for its simplicity,accurateness and reliability.更多还原