【摘要】 该文对烟叶的化学成分及其生物活性进行研究,采用硅胶,Sephadex LH-20,MCI GEL CHP 20P,HPLC等色谱技术分离纯化,根据波谱学数据(UV,IR,MS,1D和2D NMR)进行结构鉴定。从烟叶中分离鉴定了6个化合物,结构分别鉴定为顺式-5’-(2-氧代丙基)-烟碱(1),3-O-(9,12,15-octadecatrienoyl)-glyceryl-β-D-galactppyranoside(2),(l’R,2R,5S,10R)-2-(1’,2’-dihydroxy-l’-methylethyl)-6,10-dimethylspiro[4,5]dec-6-en-8-one(3),(l’S,2R,5S,10R)-2-(1’,2’-dihydroxy-l’-methylethyl)-6,10-dimethylspiro[4,5]dec-6-en-8-one(4),2,3-二羟基丙基-β-D-吡喃半乳糖苷(5),苯乙醇β-D-葡萄糖苷(6)。其中化合物1为新的生物碱,化合物2~5为首次从烟草中分离得到。对化合物1和2进行辣椒素受体(TRPV1)、褪黑素受体(MT1,MT2)的激动活性筛选,结果表明化合物1在1.53 mmol·L-1时对MT2的激动率为55.41%;化合物2在0.59 mmol·L-1时对TRPV1的激动率为128.11%,在0.73 mmol·L-1时对MT1的激动率为52.00%。更多还原

【Abstract】 In this paper,the chemical constituents and bioactivities of leaves of Nicotiana tabacum were investigated. Six compounds were isolated by means of various chromatographic techniques( silica gel,Sephadex LH-20,MCI GEL CHP-20 P and HPLC),and their structures were elucidated as cis-5’-( 2-oxopropyl)-nicotine( 1),3-O-( 9,12,15-octadecatrienoyl)-glyceryl-β-D-galactppyranoside( 2),( l’R,2R,5S,10R)-2-( 1’,2’-dihydroxy-l’-methylethyl)-6,10-dimethylspiro [4,5]dec-6-en-8-one( 3),( l’S,2R,5S,l0R)-2-( 1’,2’-dihydroxy-l’-methylethyl)-6,10-dimethylspiro [4,5] dec-6-en-8-one( 4),2,3-dihydroxypropyl-β-D-galactoside( 5) and phenylethyl β-D-glucopyranoside( 6) by extensive spectroscopic analyses( UV,IR,MS,1D-and 2D-NMR). Among them,compound 1 is a new alkaloid,and compounds 2-6 are isolated for the first time from N. tabacum. Compounds 1 and 2 were assayed for agitating activities on transient receptor potential vanilloid 1( TRPV1),melatonin receptor 1 and 2( MT1 and MT2),1 showed agitating rate of 55. 41%( 1. 53 mmol · L- 1) on MT2 and 2 possessed agitating rate of 128. 11%( 0. 59 mmol · L- 1) and 52. 00%( 0. 73mmol·L- 1) on TRPV1 and MT1,respectively.更多还原