【摘要】 目的应用骨生物力学和Micro-CT技术,探讨辅酶Q10对10月龄小鼠股骨的显微结构和生物力学的影响。方法 35只SPF级8月龄雌性KM小鼠,随机分为正常对照组(CON)、维生素E组(VE)、辅酶Q10低剂量组(Co Q10L)、辅酶Q10高剂量组(Co Q10H),辅酶Q10低剂量联合维生素E(Co Q10L+VE),连续灌胃给药10周。实验结束后,取右侧股骨进行Micro-CT扫描及三维重建,左侧股骨进行生物力学检测。结果与CON组比较,Co Q10L组小鼠右侧股骨的微观参数:体积分数、连接密度和骨密度明显增加,左侧股骨的骨生物力学刚度增加。Co Q10H体积分数、连接密度和SMI明显增加,同时骨生物力学参数中弹性载荷和最大位移得到明显增加。Co Q10L联合VE组连接密度增加,生物力学方面参数没有统计学差异。与VE比较,辅酶Q10两个剂量和辅酶Q10低剂量联合维生素E参数并没有统计学差异。结论 Co Q10(500 mg·kg-1·d-1)能够修复小鼠的股骨微观结构,提高骨生物力学的性能,修复骨量、降低股骨骨折风险的能力优于VE,提示Co Q10的抗骨质疏松作用具有良好的研究前景。更多还原

【Abstract】 Aim Bone biomechanics and micro-CT were conducted to investigate the effect of Co-enzyme Q10 on micro-structure and biological mechanics of femur in ten-month-old mice.Me thods 35 eight-month-old female KMmice in SPF grade were randomly divided into normal control group( CON) group,vitamin E group( VE),co-enzyme Q10group( Co Q10L),co-enzyme Q10group( Co Q10H),Co Q10 L + VE.Drugs were continuously given for 10 weeks.After the experiment,the femur of right side in each mouse was taken and scanned with micro-CT and 3D-reconstructed.In addition,the left femurs were used to performbio-mechanical testing.Re sults Compared with CON group,the microscopic parameters of right femur of mice in Co Q10 L group indicated that the bone volume,connection density and bone mineral density were notably increased.Whereas the bone bio-mechanical parameters of left femur showed that the stiffness were markedly increased.Co Q10 H group indicated that the bone volume,connection density and SMI were notably increased.Whereas the bone bio-mechanical parameters of left femur showed that maximum displacement and elastic loadwere markedly increased.Co Q10 L + VE group indicated that connection density was notably increased.There was no statistical difference in biomechanical parameters.Compared with VE group,the two doses of Co Q10 and Co Q10 L + VE were not significantly different.Conclusion Co Q10( 500 mg·kg-1·d-1) might repair femoral Micro-structure of mice.The effect of Co Q10 on repairing the bone mass and lowering femoral fracture risk is better than that of VE,implying that the anti-osteoporosis effect of Co Q10 is of promising prospect and should be further explored.更多还原