【摘要】 目的研究三花假卫矛（Microtropis triflora Merr.et Freem.）的化学成分。方法采用硅胶柱色谱,Sephadex LH-20凝胶柱色谱,半制备高效液相色谱仪等分离技术进行分离纯化,通过理化性质和波谱方法鉴定化合物的结构。结果从三花假卫矛的甲醇提取物中分离得到10个化合物。其结构分别鉴定为:3,25-环氧-1α,3α,11α,12,23,24-六羟基-乌苏-12-烯（1）,β-香树脂醇（2）,β-香树脂醇棕榈酸酯（3）,3β-羟基-12-齐墩果烯-11-酮-3-棕榈酸酯（4）,羽扇豆醇（5）,木栓酮（6）,3-氧-木栓烷-28-羧酸（7）,齐墩果酸（8）,大子五层龙酸（9）和直楔草酸（10）。结论化合物1为一个新的三萜类化合物,并对Bcap37及SMMC7721肿瘤细胞具有显著抑制作用,IC₅₀值分别为27.86和11.38μg·m L^-1。更多还原

【Abstract】 OBJECTIVE To study the chemical constituents of Microtropis triflora Merr.et Freem.METHODS The compounds were isolated and purified by various chromatographic technigues such as silica gel,Sephadex LH-20,and pre-HPLC.Their structures were identified on the basis of chemical properties and spectral analysis.RESULTS Ten triterpenes were isolated and elucidated as 3,25-epoxy-1α,3α,11α,12,23,25-hexahydroxy-urs-12-en（1）,β-amyrin（2）,β-amyrin palmitate（3）,3β-hydroxy-11-oxo-olean-12-enyl-3-palmitate（4）,lupeol（5）,friedelin（6）,3-oxo-28-friedelanoic acid（7）,oleanolic acid（8）,salaspermic acid（9）,and orthosphenic acid（10）.CONCLUSION Compound 1 is a new compound and has cytotoxic activity against Bcap37 and SMMC7721 cells with IC₅₀ values of 27.86 and 11.38 μg·m L^-1,respectively.更多还原