【摘要】 将热毒宁注射剂制备成鼻腔喷雾剂,进行家兔鼻腔黏膜给药药代动力学研究。以健康家兔为模型,建立空白组、鼻腔给药组和注射给药组。给药后使用乙腈沉淀蛋白法处理家兔血浆样品,HPLC检测血浆中药物有效成分绿原酸的浓度。HPLC结果显示,鼻腔给药组中可检测到绿原酸,且在给药后2~5 min时浓度最高,说明自制热毒宁鼻腔喷雾剂可成功透过鼻腔黏膜吸收进入血液中快速代谢。相比于注射给药组,鼻腔给药组血液中的药物有效物质含量较低,提示热毒宁鼻腔喷雾剂药物透黏膜吸收效率有待提高。更多还原

【Abstract】 Reduning nasal sprays were prepared from Reduning injection to explore the pharmacokinetics of its nasal administration in rabbits.Healthy rabbits were divided into three groups:blank group,nasal administration group,and intravenous injection group.Plasma samples were pretreated using acetonitrite to precipitate proteins.High-performance liquid chromatography(HPLC) was performed for the detection of chlorogenic acid.The results showed that chlorogenic acid was detected in the plasma samples of nasal administration group with the highest concentration occurring in 2-5 min after administration,which indicated that Reduning nasal sprays could be successfully absorbed from nasal mucosa and could rapidly metabolize in blood.Compared with intravenous injection group,nasal administration group had lower concentration of active ingredients,revealing that the transdermal absorption rate of Reduning nasal sprays requires further improvement.更多还原