【摘要】 目的:建立同时测定半夏糖浆中乙酸和琥珀酸含量的方法。方法:采用反相高效液相色谱法。色谱柱为GL Inter Sustain-C₁₈,流动相为0.03 mol/L磷酸二氢铵缓冲液（pH2.0）-甲醇（梯度洗脱）,流速为0.8 ml/min,检测波长为210 nm,柱温为30℃,进样量为10μl。结果:乙酸和琥珀酸检测质量浓度线性范围分别为0.020 98⁰.209 8（r=0.999 9）、12.04¹20.4μg/ml（r=0.999 9）;定量限分别为0.15、18.24 ng,检测限分别为0.045、5.53 ng;精密度、稳定性、重复性试验的RSD<2%;加样回收率分别为97.45%¹01.68%（RSD=1.39%,n=9）、98.31%¹01.08%（RSD=1.01%,n=9）。结论:该方法准确、快速,适用于同时测定半夏糖浆中乙酸和琥珀酸的含量。更多还原

【Abstract】 OBJECTIVE:To establish a method for the simultaneous determination of acetic acid and succinic acid in Banxia syrup. METHODS:RP-HPLC was performed on the column of GL Inter Sustain-C₁₈ with mobile phase of 0.03 mol/L ammonium dihydrogen phosphate buffer solution（pH2.0）-methanol（gradient elution）at a flow rate of 0.8 ml/min,the detection wavelength was210 nm,column temperature was 30 ℃,injection volume was 10 μl. RESULTS:The linear range was 0.020 98-0.209 8 μg/ml for acetic acid（0.999 9）and 12.04-120.4 μg/ml for succinic acid（r=0.999 9）;limits of quantification were 0.15,18.24 ng,limits of detection were 0.045,5.53 ng;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were97.45%-101.68%（RSD=1.39%,n=9）and 98.31%-101.08%（RSD=1.01%,n=9）. CONCLUSIONS:The method is accurate and rapid,and suitable for the simultaneous determination of acetic acid and succinic acid in Banxia syrup.更多还原