【摘要】 目的:建立藏药酸藤果的质量标准。方法:采用薄层色谱法(TLC)对药材进行定性鉴别。采用高效液相色谱法(HPLC)测定药材中酸藤果素的含量:色谱柱为Waters C18,流动相为甲醇-5%甲酸(90∶10,V/V),流速为0.9 ml/min,检测波长为288 nm,柱温为40℃,进样量为10μl。结果:酸藤果的TLC图斑点清晰、分离度好。酸藤果素检测质量浓度线性范围为4.012~40.12μg/ml(r=0.999 4);精密度、稳定性、重复性试验的RSD<2%;加样回收率为96.54%~99.57%(RSD=1.20%,n=6)。结论:该研究所建标准可用于酸藤果的质量控制。更多还原

【Abstract】 OBJECTIVE:To establish the quality standard for Embelia laeta. METHODS:TLC was adopted for the qualitative identification. HPLC was adopted for the content determination of embelin:the column was Waters C18 with mobile phase consisted of methanol-5% methanoic acid(90∶10,V/V)at a flow rate of 0.9 ml/min,the detection wavelength was 288 nm,the column temperature was 40 ℃,nd the injection volume was 10 μl. RESULTS:The TLC of E. laeta showed clear spots and good separation.The linear range of embelin was 4.012-40.12 μg/ml(r=0.999 4);RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 96.54%-99.57%(RSD=1.20%,n=6). CONCLUSIONS:The established standard can be used for the quality control of E. laeta.更多还原