【摘要】 目的:研究滋肾育胎丸对电磁辐射大鼠卵巢组织氧化损伤和卵巢颗粒细胞中核因子E2相关因子2(Nrf2)蛋白表达的影响。方法:将30只SD大鼠按体质量随机分为正常组、辐射组和辐射治疗组,每组10只。正常组大鼠不辐射,另两组置于900 MHz辐射箱中每天辐射4 h。辐射治疗组每天辐射后ig滋肾育胎丸混悬液[0.25 g(生药)/0.1 kg],正常组和辐射组ig等体积生理盐水,连续20 d后于动情期处死大鼠。采用免疫组化法检测各组大鼠卵巢颗粒细胞中Nrf2蛋白的表达,生化法检测卵巢组织总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量。结果:与正常组比较,辐射组大鼠卵巢颗粒细胞中Nrf2蛋白表达增强,卵巢组织中T-SOD和GSH-Px活性降低、MDA含量增加(P<0.05);与辐射组比较,辐射治疗组大鼠卵巢颗粒细胞中Nrf2蛋白表达无显著变化(P>0.05),卵巢组织中T-SOD和GSH-Px活性升高、MDA含量降低(P<0.05)。结论:滋肾育胎丸对900 MHz电磁辐射所致的大鼠卵巢组织氧化损伤有一定的防治作用,对Nrf2蛋白表达无明显影响。更多还原

【Abstract】 OBJECTIVE:To study the impacts of Zishenyutai pills on the ovarian tissue oxidative damage and Nrf2 protein expression in rats exposed by electromagnetic radiation(EMR). METHODS:Accordiny to their weight,30 SD rats were randomly divided into normal group,radiated group and radiated-treated group with 10 rats in each group. Normal group were not radiated,and other 2 groups were exposed by 900 MHz radiation for 4 h every day;radiated-treated group was given Zishenyutai pills suspension [0.25 g intragstrically(medicinal materials)/ml] after radiation;normal group and radiated group were given constant volume of normal saline intragastrically for consecutive 20 d. The rats were killed when they were at the estrum. The Nrf2 proteins expression of ovary were detected by immunohistochemical method,the content of ovary MDA(malondialdehyde),and activity of total superoxide dismutase(T-SOD) and superoxide dismutase(GSH-Px) were detected by biochemical method. RESULTS:Compared with the rats in normal group,the Nrf2 protein expression was higher in the rats of the radiated group;the content of ovary MDA was higher and activity of ovary T-SOD and GSH-Px were lower significantly(P<0.05). While compared with the rats in radiated group,the Nrf2 protein expression didn’t obviously change in the rats of the radiated-treated group(P>0.05),the content of ovary MDA was lower and activity of ovary T-SOD and GSH-Px were higher significantly(P<0.05). CONCLUSIONS:Zishenyutai pills can prevent and cure ovary tissue oxidative injury induced by 900 MHz radiation,but have no effect on the expression of Nrf2 protein.更多还原