【摘要】 目的:研究复方鹿角乳痛宁胶囊对乳腺增生大鼠的改善作用及其机制。方法:120只大鼠随机均分为正常组、模型组、他莫昔芬(阳性药物,1.8 mg/kg)组和复方鹿角乳痛宁胶囊低、中、高剂量(70、140、280 mg/kg)组。除正常组外,各组大鼠均im苯甲酸雌二醇和黄体酮以诱导乳腺增生模型;模型复制成功后ig相应药物,正常组和模型组ig等体积蒸馏水,每天1次。30 d后观察复方鹿角乳痛宁胶囊对大鼠乳头直径的影响,测定大鼠血清中血清促乳素(PRL)、促卵泡生成素(FSH)、雌二醇(E2)、黄体生成素(LH)、孕酮等的水平,并观察大鼠乳腺组织形态学变化。结果:与造模前比较,造模后给药前各组大鼠乳头直径均增加(P<0.05)。给药后,与模型组比较,各给药组大鼠乳头直径减小;复方鹿角乳痛宁胶囊中、高剂量组大鼠血清中PRL、FSH、E2水平降低,LH、孕酮水平升高,低剂量组FSH水平升高、孕酮水平降低(P<0.05);各给药组大鼠乳腺组织病变不同程度减轻,乳腺小叶个数、腺泡个数及导管上皮层数不同程度地减少(P<0.05),形态改善。复方鹿角乳痛宁胶囊中、高剂量组与阳性药物组药效相当。结论:复方鹿角乳痛宁胶囊对乳腺增生大鼠有一定的改善作用;其机制可能与降低血清中PRL、FSH、E2水平,升高血清中LH、孕酮水平有关。更多还原

【Abstract】 OBJECTIVE:To study the effects and mechanism of Compound lujiao rutongning capsule on rats with mammary glands hyperplasia. METHODS:120 rats were randomly divided into normal group,model group,tamoxifen group(positive drug,1.8 mg/kg)and Compound lujiao rutongning capsule low-dose,middle-dose and high-dose groups(70,140,280 mg/kg).The model of mammary gland hyperplasia was induced by injecting benzoate estradiol and progesterone except normal group. After modeling,they were given relevant medicines intragastrically,and normal group and model group were given isometric distilled water intragastrically once a day. The effects of Compound lujiao rutongning capsule on the diameters of nipple in rats were observed after 30 d. The levels of PRL,FSH,E2,LH and progesterone in serum of rats were all determined,and morphology of breast tissue was observed. RESULTS:Compared with before modeling,the diameters of nipple in rats increased before medication and after modeling(P<0.05). After medication,compared with model group,the diameters of nipple in rats decreased in treatment groups,and the serum levels of PRL,FSH and E2 in Compound lujiao rutongning capsule medium-dose and high-dose groups decreased,while the levels of LH and progesterone increased;the level of FSH in Compound lujiao rutongning capsule low-dose group increased,while the level of progesterone decreased(P<0.05). Breast tissue lesions of rats relieved to different extent,the number of lobuli mammae and acinus and the layer of duct epithelial decreased to different extent(P<0.05),and morphology of breast tissue had been improved. The effect of Compound lujiao rutongning capsule was equal to positive drug. CONCLUSIONS:Compound lujiao rutongning capsule could ameliorate mammary glands hyperplasia of rats,and its mechanism may be associated with the down-regulation of PRL,FSH and E2 and the up-regulation of LH and progesterone.更多还原