【摘要】 动物源性成分鉴定被广泛应用于食品和饲料以及多种生物制品的检测中,现有检测方法中基于16S rRNA基因的通用引物由于存在与部分物种的mt DNA序列错配,导致检测中可能会出现假阴性。本方法对16S rRNA引物进行了改进,提高了引物与多个物种mt DNA序列的匹配度,使得检测的特异性提高,适用范围更广。对近20种动物源性成分的检测显示均能获得良好的检测效果,同时还能根据产物大小初步判断动物种类,提高了检测的效率和准确性。更多还原

【Abstract】 The identification of animal-derived ingredients was widely used in food adulteration and animal forage safety. The existed primers designed for 16S rRNA gene were widely used for detection of animal-derived ingredients,but the sequence of upstream primer had a number of 3 ~6 mismatched bases with some species, which resulted in false negative results. The modification of this primer sequence decreased the number of mismatched bases that could improve the PCR specificity in identification. The modified primers had higher efficiency to about 20 species in animal derived ingredients detection. Furthermore, the lengths of PCR products were different in several species, which could be as a preliminary criterion for species identification. The modification of this primer improved the efficiency and accuracy in the field of authentication of animal-derived ingredients.更多还原