【摘要】 目的:探讨茶多酚对力竭运动大鼠氧化应激和炎症因子的影响,为减少运动损伤寻找有效的干预手段。方法:24只雄性SD大鼠按体重随机分为对照组(C)、力竭组(E)和茶多酚力竭组(F,300mg/kg/d茶多酚灌胃),4周后,进行一次力竭游泳运动,测定大鼠血清肌酸激酶(CK)、乳酸脱氢酶(LDH)、丙二醛(MDA)以及炎症细胞因子白细胞介素(IL-1β,IL-10)和肿瘤坏死因子-α(TNF-α)含量。结果:与C组相比,E组大鼠力竭运动后血清CK、LDH活性和MDA含量显著升高(P<0.05);促炎因子TNF-α和抗炎因子IL-10水平显著增加(P<0.01和P<0.05)。与E组相比,F组大鼠血清CK、LDH活性和MDA含量显著降低(P<0.05);促炎因子IL-1β和TNF-α水平显著下降(P<0.05),而IL-10/TNF-α比值显著升高(P<0.05)。相关性分析显示,MDA与CK和TNF-α之间存在显著正相关(P<0.05),与IL-10/TNF-α比值呈非常显著负相关(P<0.01)。结论:茶多酚补充能提高机体抗氧化状态,减轻炎症反应,对力竭运动的大鼠骨骼肌具有保护作用。更多还原

【Abstract】 Objectives:To study the effects of tea polyphenols supplemnet on oxidative stress and inflammatory response in rats after exhaustive exercise,find out effective interventions for reducing the sports injuries.Methods:Twenty-four male SD rats were randomly divided into three groups according to weight:control group(C),exhaustive exercise group(E),exhaustive exercise with Tea polyphenols(F,dose:300mg/kg/d).4 weeks later,the rats performed once exhaustive exercise,and the serum creatine kinase(CK),lactate dehydrogenase(LDH),malondialdehyde(MDA),the level of inflammatory cytokine:interleukin(IL-1β,IL-10)and the tumor necrosis factor alpha(TNF-a)were examined.Results:Compared with C group,the serum CK and LDH activity and MDA content of E group rats were significantly increased after exhaustive exercise(P<0.05);also,the levels of pro-inflammatory cytokine TNF-a and anti-inflammatory cytokine IL-10 were significantly increased(P<0.01 and P<0.01).Compared with E group,the serum CK and LDH activity and MDA content of F group rats significantly decreased(P<0.05);the levels of pro-inflammatory Cytokine TNF-a and IL-1β were all significantly decreased(P<0.05),whereas the ratio of IL-10/TNF-α was significantly improved(P<0.05).Correlation analysis showed that it appeared positive correlation MDA with CK and TNF-a(P<0.05),but with ratio of IL-10/TNF-a,they appeared very significantly negative correlation(P<0.01).Conclusion:The supplement of tea polyphenols can improve the body’s antioxidant status,reduce inflammation response and play aprotective role to skeletal muscle in rat performing exhaustive exercise.更多还原