【摘要】 目的探讨氧化苦参碱（OXY）对高脂诱导IR ApoE^-/-小鼠肝脏成纤维细胞因子-21（FGF-21）/β-klotho/FGF-21受体1（FGFR1）通路的作用。方法 ApoE^-/-小鼠分为模型（IR）组、氧化苦参碱25 mg/kg（IR+25OXY）组、氧化苦参碱50 mg/kg（IR+50OXY）组、氧化苦参碱100 mg/kg（IR+100OXY）组,C57BL/6J小鼠作为对照（NC）组。计算HOMA-IR和ISI。QRT-PCR和Western blot检测肝组织FGF-21、β-klotho蛋白（β-klotho）、FGFR1表达。结果与IR组比较,IR+50OXY组和IR+100OXY组HOMA-IR值降低[（31.54±4.30）vs（21.84±1.87）vs（17.27±3.59）],ISI值升高[（0.022±0.000）vs（0.034±0.010）vs（0.042±0.010）]（P<0.05）;OXY可使FGF-21、β-klotho、FGFR1 mRNA和蛋白表达有不同程度的降低。结论 OXY能通过调节肝脏FGF-21/β-klotho/FGFR1通路,改善高脂诱导小鼠的IR。更多还原

【Abstract】 Objective To investigate the effect of oxymatrine（OXY） on hepatic FGF-21 /β-klotho/FGFR1 pathway in ApoE^-/- mice with fat-induced insulin resistance.Methods ApoE^-/- mice were divided into model group（IR）,oxymatrine 25 mg/kg group（IR + 25OXY）,oxymatrine 50 mg/kg group（IR+50OXY）,and oxymatrine 100 mg/kg group（IR+ 100OXY）.C57BL/6J mice were selected as normal control group（NC）.Insulin resistance index（HOMA-IR） and insulin sensitivity index（ISI） were calculated.The expression of fibroblast growth factor 21（FGF-21）,β-klotho protein family（β-klotho） and FGF-21 receptor 1（FGFR1） were examined by QRT-PCR and Western-blot.Results HOMA-IR decreased in IR+50 OXY group and IR+100 OXY group than in IR group[（21.84 ± 1.87） vs（17.27 ±3.59） vs（31.54 ± 4.30）,P<0.05].ISI increased in IR+50 OXY group and IR+100 OXY group than in IR group[（0.034 ± 0.010） vs（0.042 ± 0.010） vs（0.022 ± 0.000）,P<0.05].Oxymatrine reduced the mRNA and protein expression of FGF-21,β-klotho and FGFR1 in different degree.Conclusion Oxymatrine can improve fat-induced insulin resistance in mice by modulating the hepatic FGF-21/β-klotho/FGFR1 pathway.更多还原