【摘要】 细菌的生物被膜是病原微生物产生耐药性、免疫逃逸和造成持续感染的重要因素之一。为了解抗菌肽HJH-1对猪胸膜肺炎放线杆菌形成生物被膜的影响,本研究采用微量测定法结合扫描电子显微镜技术来评价HJH-1对APP生物被膜的作用。结果显示,与对照组相比,添加浓度为100 g/m L的抗菌肽HJH-1后,在培养APP至第48小时形成的生物被膜的数量显著减少,说明HJH-1能显著抑制APP的生长(P<0.05);扫描电子显微镜的检测结果也显示,HJH-1在50 g/m L时APP形成的生物被膜变薄,细菌的间隙增大,HJH-1在100 g/m L时可明显抑制APP形成生物被膜;小鼠肺炎模型研究结果也发现,皮下注射HJH-1(20 mg/kg)可以有效减轻APP所引起的肺部炎症性反应。本研究结果为猪胸膜肺炎放线杆菌的治疗提供新的思路,也为抗菌肽HJH-1的临床应用提供了理论基础。更多还原

【Abstract】 Bacterial biofilm is one of the most important factors causing resistant pathogenic microorganisms,immune escape and persistent infections.Polystyrene micro assay combined with scanning electron microscopy(SEM) were used to evaluate the impact of antimicrobial peptides HJH-1 on the biofilm formation of Actinobacillus pleuropneumoniae.Our results showed HJH-1 can significantly inhibit the biofilm formation of APP within 48 h,more importantly,can inhibit the bacterial growth of APP(P<0.05) at the density of 100 g/m L.SEM results showed the treatment of HJH-1 at 50 g/m L led to thinner biofilm formation of APP and increased bacterial interspace,the treatment of HJH-1 at 100 g/m L can significantly inhibit the biofilm formation of APP,pneumonia mouse model indicated that HJH-1(20 mg/kg) could effectively alleviate the APP-induced pneumonia.This study not only provides a reference for Actinobacillus pleuropneumoniae treatment,but also provides a new idea for the clinical application of antimicrobial peptides HJH-1.更多还原