Freeze-tolerant yeasts are required for the production of frozen doughs. The authors developed a freezetolerant yeast strain AFY-1 with a method of low-temperature plasma mutation combined with orient domestication and screening, using Saccharomyces cerevisiae CGMCC 2.1423 as the parent. In the present study, the strain homology of freeze-tolerant yeast and S. cerevisiae was identified with a molecular-biological method. The cell survival rate and the fermentability of freeze-tolerant yeast in freezing stor...