【摘要】 通过定性PCR技术,对棉籽壳培养基及栽培银耳中的转基因成分进行检测。以棉籽壳培养基及栽培银耳的DNA为模板,设置棉花内源基因Sad1内参对照、转基因棉籽壳阳性对照、非转基因棉籽壳阴性对照和无菌双蒸水空白对照,利用不同的引物分别对CaMV35S启动子、NOS终止子以及Cry1A抗虫基因的特异片段进行PCR扩增及电泳检测。在棉籽壳培养基中可检测出35S、NOS及Cry1A的特异片段,而在棉籽壳栽培的银耳中仅检测出35S和NOS的特异片段,未检出Cry1A的特异片段。研究结果表明,棉籽壳培养基中含有外源转基因成分,并向银耳发生水平转移,而其外源抗虫基因可能未转入银耳中。对棉籽壳栽培银耳中基因水平转移的影响因素和潜在生物安全性进行了讨论。更多还原

【Abstract】 In this paper, genetically modified(GM) elements were detected and analyzed in the cottonseed hull medium and the cultivated tremella by qualitative polymerase chain reaction(PCR) technology. Setting the cotton endogenous gene Sad1 as the internal control, the transgenic cottonseed hulls as the positive control, the non-transgenic cottonseed hulls as the negative control and sterile double distilled water as the blank, using the DNA extracted from the cottonseed hull medium and the cultivated tremella as a template, fragments were amplified and detected by electrophoresis specific for Ca MV35 S promoter, NOS terminator and insecticidal gene Cry1 A, respectively. Thre results showed that 35 S, NOS and Cry1A-specific fragments were detected in the cottonseed hull medium. However, only 35 S and NOS-specific fragments were detected in the cottonseed hull cultivated tremella, Cry1A-specific fragment not detected.. The results indicated that the cottonseed hull medium contains genetically modified(GM) elements, and it was probably horizontally transferred into the cultivated tremella, but the exogenous insecticidal gene might not be transferred.Factors probably affecting horizontal gene transfer(HGT) in the cottonseed hull cultivated tremella and its potential biosafety were discussed in this paper.更多还原