【摘要】 目的:遥测海洛因诱导条件性位置偏爱大鼠穿梭觅药潜伏期边缘前区(Pr L)的局部场电位(LFPs),探索与觅药动机形成相关的神经振荡改变。方法:将Pr L区埋藏电极的大鼠随机分为手术对照组和海洛因诱导CPP组。利用视频监视和脑电无线遥测技术,记录大鼠黑-白箱穿梭觅药过程中的实时LFPs,通过快速傅立叶变化和小波包提取技术对原始LFPs进行分析。结果:与手术对照组比较,海洛因诱导CPP组大鼠黑-白箱穿梭潜伏期左右侧θ节律百分比增加,左侧γ3节律百分比增加,出现θ~γ3相位振幅耦合增强。MK-801微量注射Pr L区后,大鼠觅药行为明显减少,左右侧θ节律百分比降低,左侧θ~γ3相位振幅耦合减弱。结论:本研究提示海洛因诱导CPP组大鼠Pr L区θ振荡活动增强,左侧θ~γ3相位振幅耦合增强,可能与大鼠海洛因觅药动机和行为发动密切相关,这种电活动的改变也与Pr L区谷氨酸能神经元及其受体的功能密不可分。更多还原

【Abstract】 Objective: To investigate the relationship between the changes of neural oscillations and the drug-seeking motivation,record the telemetric local field potentials( LFPs) of the prelimbic cortex( Pr L) in the latency of drug-seeking behavior of conditioned place preference( CPP) rats induced by heroin. Methods: The recording electrode was stereotactically implanted into the Pr L cortex of rats. The animals were then randomly divided into operation-only control and heroin-induced CPP groups,respectively. A CPP video system in combination with a wireless telemetry device was used for recording LFPs when the rats shuttled between black-white chamber for drug-seeking. The LFPs were analyzed by fast Fourier transform( FFT) and wavelet packet extraction. Results: Compared with operation-only control group,the LFPs recorded in Pr L area of heroin-induced CPP group of rat during black-white chamber shuttling showed that the percentage of θ rhythm were increased in right and left Pr L,the percentage of γ3 rhythm was increased in left Pr L,the phase-amplitude coupling of θ and γ3 was increased in left Pr L. After MK-801 Maleate micro injection to Pr L area,drug-seeking behavior of rat was markedly reduced and the percentage of θ oscillation was depressed,the phase-amplitude coupling of θ and γ3 was depressed in left Pr L compared with operation-only control group. Conclusion: These results suggest that increase of θ oscillations of Pr L area may be related to the drug-seeking motivation and behavior launching in heroin-induced CPP groups of rat. The changes of θ oscillations also have close relationship with glutamatergic neuron and the receptor of it on Pr L area.更多还原