【摘要】 目的:观测大鼠骨骼肌钝挫伤(SMBI)恢复进程中腺苷酸活化蛋白激酶α2(AMPKα2)、缺氧诱导因子-1α(HIF-1α)的表达变化,探讨SMBI恢复可能生物学机制。方法:雄性Wistar大鼠48只,随机选取6只作为正常对照组;另42只大鼠重物砸伤后肢小腿建立钝挫伤模型后分7组(n=6),造模后各组分别在伤后12 h、2 d、5 d、7d、10 d、15 d、30 d取材,检测小腿三头肌HIF-1α、AMPKα2表达的变化。结果:损伤后12 h HIF-1α、AMPKα2表达均明显升高,在伤后15 d开始回落接近正常;HIF-1α、AMPKα2表达的峰值出现在伤后2 d内,伤后5 d后表达量开始下降;除HIF-1αm RNA在伤后2 d组表达出现峰值外,其余时间点二者m RNA与蛋白表达时程变化基本一致。结论:SMBI后,HIF-1α、AMPKα2可能通过参与调解缺氧适应、肌细胞再生、能量代偿起到促进伤后恢复的作用。更多还原

【Abstract】 Objective: To explore the possible biological mechanisms of skeletal muscle injury recovery by observing the changes of AMP-activated protein kinase α2( AMPKα2) and hypoxia inducible factor-1α( HIF-1α) expression levels of rats in the skeletal muscle blunt injury recovery process. Method: Six of the 48 male Wistar rats was randomly selected as the normal control group. The blunt injury model was set up by injuring the hind legs of remaining 42 rats with heavy objects. Then they were divided into 7 groups(n = 6). The changes of AMPKα2 and HIF-1α expression levels were tested in the hind limb triceps surae of the model rats from each of the 7 groups at 12 hours,2 days,5 days,7 days,10 days,15 days and 30 days respectively following the injury. Results: The expression levels of AMPKα2 and HIF-1α all increased significantly at 12 hours following the injury and fell close to normal levels at 15 days following the injury. The values of AMPKα2 and HIF-1α expression peaked within 2 days after injury and the expression levels began to decline at 5 days after injury. Except the peak,the changes of the m RNA expressions of the two proteins were basically consistent with those of protein expression at the other time points. Conclusion: HIF-1α and AMPKα2 might play roles in mediating hypoxia adaptation,muscle cell regeneration,and energy compensation to promote recovery after injury.更多还原