【摘要】 目的:采用2.5%葡聚糖硫酸钠(DSS)定量灌胃诱导小鼠溃疡性结肠炎(UC),观察小鼠结肠通透性改变与肿瘤坏死因子α(TNF-α)及NF-κB p65的关系。方法:48只ICR小鼠随机分为2组(n=24):对照组和模型组。模型组小鼠给予2.5%DSS定量灌胃诱发小鼠急性UC,对照组小鼠予同体积的蒸馏水灌胃代替。记录两组小鼠疾病活动指数(DAI),9 d后测定两组小鼠结肠组织病理学评分、结肠通透性、TNF-α及NF-κB p65。统计分析DAI、结肠通透性、TNF-α与NF-κB p65之间的相关性。结果:与对照组比较,模型组小鼠DAI、结肠病理学评分、结肠通透性、TNF-α、NF-κB p65均显著增高(P均<0.01)。小鼠DAI增高与结肠通透性密切相关(P均<0.01),结肠通透性增高与TNF-α、NF-κB p65密切相关(P均<0.01)。结论:与对照组小鼠相比,DSS造模小鼠的结肠通透性显著增高,并与TNF-α、NF-κB p65增高呈正相关。TNF-α、NF-κB p65增高导致结肠通透性增高,进而导致炎症免疫反应过度增强,可能是UC发病的重要环节。更多还原

【Abstract】 Objective:To study the changes of colonic permeability and its correlation with TNF-α,NF-κB p65 in indextran sulphate sodium(DSS)– induced ulcerative colitis(UC)of mice. Methods:Forty-eight ICR mice were randomly divided into the control group and the model group. The acute UC model was induced by quantified intragastric administration of 2. 5% DSS in mice. The disease activity index(DAI),histopathology scores,colonic permeability,expression of TNF-α,NF-κB p65 in colonic tissue were determined. The change of colonic permeability and its correlation with DAI,TNF-α,NF-κB p65 were analyzed. Results:Compareded with the control group,DAI colonic permeability of colonic tissue,and the expression of TNF-α NF-κB p65 in the model group were increased significantly(P < 0. 01,P < 0. 01). The increased colonic permeability correlated with DAI(P < 0. 01),and the expression of TNF-α(P < 0. 01),NF-κB p65(P < 0. 01)changed significantly. Conclusion:The alteration of colonic permeability and increased expression of TNF-α,NF-κB p65 may play important roles in the occurrence and development of UC.更多还原