【摘要】 目的通过检测喘息患儿FcεRI-β基因多态性与临床表现、药物疗效及哮喘相关性的研究,探讨该基因多态性对喘息患儿的易感性、疗效与预后的作用。方法选取住院喘息患儿146例,用荧光定量PCR法检测患儿FcεRI-β基因,其中AG、GG为风险基因型,共41例,AA为正常基因型,共105例;记录两组患儿的喘息发作程度、检测肺功能;记录两组临床疗效(雾化治疗、全身使用糖皮质激素、住院时间、后续治疗);出院后随访2年,记录两组患儿的哮喘发病率。结果风险型基因组与正常型基因组在喘息发作严重程度中的分布有统计学差异(P=0.003),风险型基因组较正常型基因组喘息发作严重;两组患儿在肺功能异常上无统计学差异(P=0.53);两组在雾化治疗上无统计学差异(P=0.74);两组在使用全身糖皮质激素上无统计学差异(P=0.21);两组在住院时间上无统计学差异(P=0.21);两组患儿在后续治疗上没有统计学差异,P=0.63>0.05;两组患儿在随访2年后,哮喘发病率上无统计学差异(P=0.13)。结论 FcεRI-β基因多态性与患儿的喘息发病相关,风险型基因是喘息发生的危险因素;风险型基因影响喘息发作的严重程度;但不是影响肺功能的主要因素;基因多态性不是影响疗效的主要因素;也不是发生哮喘的主导因素。更多还原

【Abstract】 Objective To study the correlation between FcεRI- β gene polymorphism,clinical manifestations,curative effect,and asthma of wheezing infants,explore the effect of FcεRI- β gene polymorphism on susceptibility,curative effect,and prognosis of wheezing infants. Methods A total of 146 hospitalized wheezing infants were selected,fluorescence quantitative PCR was used to detect FcεRI- βgene,41 cases with risk genotypes( AG,GG) and 105 cases with normal genotype( AA) were included. The degrees of asthma in the two groups were recorded,pulmonary function was detected. The clinical effects in the two groups were recorded,the indexes included atomization treatment,systemic treatment of glucocorticoid,hospitalization time,and subsequent treatment. The cases were followed up for two years after leaving the hospital,the incidence rates of asthma in the two groups were recorded. Results The degree of asthma in risk genotype group was statistically significantly severer than that in normal genotypes group( P = 0. 003); there was no statistically significant difference in pulmonary function,atomization treatment,systemic treatment of glucocorticoid,hospitalization time,subsequent treatment and incidence rate of asthma during follow- up between the two groups( P = 0. 53,P = 0. 74,P = 0. 21,P = 0. 21,P = 0. 63 > 0. 05,P = 0. 13). Conclusion FcεRI- β gene polymorphism is correlated with asthma,risk genotypes are risk factors of asthma,which affect the severity of asthma,but they are not the main influencing factors of pulmonary function. Gene polymorphism is not the main influencing factor of curative effect and leading factor of occurrence of asthma.更多还原