【摘要】 目的探讨核转录因子KLF7与脂肪源性干细胞(ADSC)联合应用对小鼠脱细胞同种异体神经支架(ANA)移植坐骨神经缺损后轴突再生和功能恢复的影响。方法成年C57BL/6小鼠随机分为脱细胞神经支架(ANA)组、ADSC组和KLF7+ADSC组,每组10只。坐骨神经功能指数(SFI)和电生理方法检测神经运动功能的恢复,Western bolt检测神经移植体内KLF7、Trk A和Trk B的蛋白表达。NF200免疫荧光法检测神经支架中轴突再生,并示踪PKH26标记的移植ADSC。结果与ADSC组比较,KLF7+ADSC组神经移植体内KLF7、Trk A和Trk B蛋白表达明显增高,NF200表达增强,PKH-26标记的ADSC数量显著增高(P<0.05)。与ANA组比较,ADSC组和KLF7+ADSC组电生理波幅增高、神经传导速度增快、延迟期缩短,SFI功能指数增高,其中KLF7+ADSC组神经恢复作用显著优于ADSC组(P<0.05)。结论 KLF7联合ADSC移植对小鼠ANA修复坐骨神经缺损后轴突再生和功能恢复的作用优于ADSC组,其机制可能与KLF7高表达促进神经移植体内Trk A和Trk B表达,进而促进移植的ADSC存活有关。更多还原

【Abstract】 Objective To study the effect of KLF7 and adipose-derived stem cell(ADSC) transplantation on expression of axonal regeneration and function recovery after acellular nerve allograft(ANA) repair of the sciatic nerve gap in mice.Methods Adult C57 BL/6 mice were randomly divided into ANA group,ADSC group and KLF7 + ADSC group.The sciatic functions index(SFI) and electrophysiology were used to evaluate functional recovery.KLF7,Trk A and Trk B protein were evaluated by Western bolt,the protein expression of NF200 and the fluorescence signal of ADSC labeled with PKH-26 in the nerve graft was observed by using immunofluorescence.Results Compared with ANA group,the expression of KLF7,Trk A,Trk B,NF200 and PKH-26 labeled ADSC in KLF7 + ADSC group were increased(P < 0.05).Compared with ANA group,nerve conduction velocity,wave amplitude and the SFI score were significantly increased in ADSC group and KLF7 + ADSC group(P < 0.05),however,incubation period were significantly decreased in two treatment groups(P <0.05),and the effect of KLF7 + ADSC group was more powerful than ADSC group(P < 0.05).Conclusion The combined KLF7 and ADSC transplantation treatment promoted axon regeneration and function recovery much significantly than ADSC treatment,which may be related to KLF7 upregulating Trk A and Trk B expression in the ANA,and promoting ADSC survival.更多还原